caa a22 4500 469045183 CHVBK 20180323132822.0 cr unu---uuuuu 170328e19920301xx s 000 0 eng 10.1007/BF01799639 doi (NATIONALLICENCE)springer-10.1007/BF01799639 Diminished concentration of the NF-H subunit of neurofilaments in cerebral cortex of rats chronically treated with proline, methylmalonate and phenylalanine plus α-methylphenylalanine [Elektronische Daten] [M. Rubin, C. Wannmacher, G. Valente, M. Camargo, R. Pureur] Summary: Wistar rats from the same litter were randomly divided into four groups and received subcutaneously from the 6th to 28th daypost partum one of the following drugs:l-proline, methylmalonate,l-phenylalanine plus α-methylphenylalanine, or equivalent volumes of 0.9% (w/v) saline (controls). On day 30, the animals were killed, the brain was removed and the cerebral cortex and cerebellum was immediately dissected. Total intermediate filament fraction (IF) was obtained from cerebral cortex and cerebellum by using a high-salt phosphate-buffered solution supplemented by 1% Triton X-100. The pellet contained the bulk of the IF proteins. Following SDS-polyacrylamide gel electrophoresis, these proteins were identified as the 200, 150 and 68 kD subunits of neurofilaments (NF-H, NF-M and NF-L, respectively), the 66 kDa associated protein, the 57 kDa intermediate filament-like protein and the 52 kDa glial fibrillary acidic protein (GFAP). They were further scanned through densitometry from enriched fractions of controls and of animals treated with the various drugs in order to determine the effects of the treatments on their concentration. Our results showed that the concentration of IF protein in cerebellum was not affected by the treatments, whereas chronic administration of all drugs significantly decreased NF-H subunit concentration in rat cerebral cortex. SSIEM and Kluwer Academic Publishers, 1992 Rubin M. Departamento de Bioquimica, Instituto de Biociências, UFRGS, Rua Sarmento Leite, 500, 90050, Porto Alegre - RS, Brasil aut Wannmacher C. Departamento de Bioquimica, Instituto de Biociências, UFRGS, Rua Sarmento Leite, 500, 90050, Porto Alegre - RS, Brasil aut Valente G. Departamento de Bioquimica, Instituto de Biociências, UFRGS, Rua Sarmento Leite, 500, 90050, Porto Alegre - RS, Brasil aut Camargo M. Departamento de Bioquimica, Instituto de Biociências, UFRGS, Rua Sarmento Leite, 500, 90050, Porto Alegre - RS, Brasil aut Pureur R. Departamento de Bioquimica, Instituto de Biociências, UFRGS, Rua Sarmento Leite, 500, 90050, Porto Alegre - RS, Brasil aut Journal of Inherited Metabolic Disease Kluwer Academic Publishers 15/2(1992-03-01), 252-260 0141-8955 15:2<252 1992 15 10545 https://doi.org/10.1007/BF01799639 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01799639 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rubin M. Departamento de Bioquimica, Instituto de Biociências, UFRGS, Rua Sarmento Leite, 500, 90050, Porto Alegre - RS, Brasil aut NATIONALLICENCE 700 1- Wannmacher C. Departamento de Bioquimica, Instituto de Biociências, UFRGS, Rua Sarmento Leite, 500, 90050, Porto Alegre - RS, Brasil aut NATIONALLICENCE 700 1- Valente G. Departamento de Bioquimica, Instituto de Biociências, UFRGS, Rua Sarmento Leite, 500, 90050, Porto Alegre - RS, Brasil aut NATIONALLICENCE 700 1- Camargo M. Departamento de Bioquimica, Instituto de Biociências, UFRGS, Rua Sarmento Leite, 500, 90050, Porto Alegre - RS, Brasil aut NATIONALLICENCE 700 1- Pureur R. Departamento de Bioquimica, Instituto de Biociências, UFRGS, Rua Sarmento Leite, 500, 90050, Porto Alegre - RS, Brasil aut NATIONALLICENCE 773 0- Journal of Inherited Metabolic Disease Kluwer Academic Publishers 15/2(1992-03-01), 252-260 0141-8955 15:2<252 1992 15 10545 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer