caa a22 4500 469045272 CHVBK 20180323132822.0 cr unu---uuuuu 170328e19920301xx s 000 0 eng 10.1007/BF01799633 doi (NATIONALLICENCE)springer-10.1007/BF01799633 Tris discriminates between the different α-glucosidase activities from extracts of human neutrophils [Elektronische Daten] [M. Ortiz de Apodaca, E. Fernandez, G. de la Fuente] Summary: In an attempt to detect acid maltase deficiency in neutrophils from patients with type II glycogenosis, without interference from the ‘renal' α-glucosidase activity present in these cells, we have evaluated the contribution of the renal component in the total activity measured at pH 4.0 in extracts of human neutrophils. The renal contribution is about 13-25% and renal glucosidase appears to be closely related to the enzyme present on the epithelium of small intestine, which is known to be inhibited by Tris. We have used this compound as a selective inhibitor of the renal component of α-glucosidase activity measured at pH 4.0 in total extracts of neutrophils. Our results demonstrate that 0.1 mol/L Tris is an inhibitor of the renal α-glucosidase present in neutrophils and can be used to reduce the interference from this enzyme in assays of acid maltase. SSIEM and Kluwer Academic Publishers, 1992 Ortiz de Apodaca M. Laboratorio de Bioquímica, Hospital Virgen de la Salud, Avda Barber s/n, 45004, Toledo, Spain aut Fernandez E. Laboratorio de Bioquímica, Hospital Virgen de la Salud, Avda Barber s/n, 45004, Toledo, Spain aut de la Fuente G. Departamento de Bioquímica, Facultad de Medicina de la U. Autónoma de Madrid, Instituto de Investigaciones Biomédicas del CSIC, 28029, Madrid, Spain aut Journal of Inherited Metabolic Disease Kluwer Academic Publishers 15/2(1992-03-01), 213-219 0141-8955 15:2<213 1992 15 10545 https://doi.org/10.1007/BF01799633 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01799633 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ortiz de Apodaca M. Laboratorio de Bioquímica, Hospital Virgen de la Salud, Avda Barber s/n, 45004, Toledo, Spain aut NATIONALLICENCE 700 1- Fernandez E. Laboratorio de Bioquímica, Hospital Virgen de la Salud, Avda Barber s/n, 45004, Toledo, Spain aut NATIONALLICENCE 700 1- de la Fuente G. Departamento de Bioquímica, Facultad de Medicina de la U. Autónoma de Madrid, Instituto de Investigaciones Biomédicas del CSIC, 28029, Madrid, Spain aut NATIONALLICENCE 773 0- Journal of Inherited Metabolic Disease Kluwer Academic Publishers 15/2(1992-03-01), 213-219 0141-8955 15:2<213 1992 15 10545 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer