caa a22 4500 469048964 CHVBK 20180323132824.0 cr unu---uuuuu 170328e19920101xx s 000 0 eng 10.1007/BF00164754 doi (NATIONALLICENCE)springer-10.1007/BF00164754 Superoxide anion production by rat neutrophils at various stages of bleomycin-induced lung injury [Elektronische Daten] [Elizabeth Tarnell, Bonnie Oliver, George Johnson, Florence Watts, Roger Thrall] This study investigated the level of activation of neutrophils isolated from rats at various stages of bleomycin-induced lung injury. Neutrophils were collected from blood and bronchoalveolar lavage (BAL) fluid and their superoxide anion (O f2 p− )-generating capacity measured in response to phorbol myristate acetate (PMA) and opsonized zymosan (OZ) stimulation. When stimulated with PMA, BAL neutrophils isolated from animals 3 days after bleomycin treatment had a significantly greater capacity to produce O f2 p− than BAL neutrophils from animals 7 days after bleomycin treatment. The O f2 p− levels of 7 day BAL neutrophils more closely resembled the resting levels obtained with circulating neutrophils from both control and bleomycin-treated animals. There were no differences observed in any of the neutrophils when stimulated with OZ. Myeloperoxidase levels were measured in plasma and BAL and found to be elevated only in plasma at 7 days after bleomycin. These data demonstrate that neutrophil activation does occur in this model and that the activation appears to be transient, in response to specific stimuli and compartmentalized between the lung and blood. Springer-Verlag, 1992 Superoxide nationallicence Neutrophils nationallicence Bleomycin-induced lung injury nationallicence Tarnell Elizabeth Department of Medicine, Pulmonary Division, University of Connecticut Health Center, 06030, Farmington, CT, USA aut Oliver Bonnie Department of Medicine, Pulmonary Division, University of Connecticut Health Center, 06030, Farmington, CT, USA aut Johnson George Department of Pediatrics, University of Connecticut Health Center, 06030, Farmington, CT, USA aut Watts Florence Department of Anesthesiology, University of Connecticut Health Center, 06030, Farmington, CT, USA aut Thrall Roger Department of Medicine, Pulmonary Division, University of Connecticut Health Center, 06030, Farmington, CT, USA aut Lung Springer-Verlag 170/1(1992-01-01), 41-50 0341-2040 170:1<41 1992 170 408 https://doi.org/10.1007/BF00164754 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00164754 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tarnell Elizabeth Department of Medicine, Pulmonary Division, University of Connecticut Health Center, 06030, Farmington, CT, USA aut NATIONALLICENCE 700 1- Oliver Bonnie Department of Medicine, Pulmonary Division, University of Connecticut Health Center, 06030, Farmington, CT, USA aut NATIONALLICENCE 700 1- Johnson George Department of Pediatrics, University of Connecticut Health Center, 06030, Farmington, CT, USA aut NATIONALLICENCE 700 1- Watts Florence Department of Anesthesiology, University of Connecticut Health Center, 06030, Farmington, CT, USA aut NATIONALLICENCE 700 1- Thrall Roger Department of Medicine, Pulmonary Division, University of Connecticut Health Center, 06030, Farmington, CT, USA aut NATIONALLICENCE 773 0- Lung Springer-Verlag 170/1(1992-01-01), 41-50 0341-2040 170:1<41 1992 170 408 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer