caa a22 4500 469049227 CHVBK 20180323132825.0 cr unu---uuuuu 170328e19920901xx s 000 0 eng 10.1007/BF00566679 doi (NATIONALLICENCE)springer-10.1007/BF00566679 Intercellular adhesion molecule-1 contributes to pulmonary oxygen toxicity in mice: Role of leukocytes revised [Elektronische Daten] [Craig Wegner, Walter Wolyniec, April LaPlante, Kristin Marschman, Klaus Lubbe, Nancy Haynes, Robert Rothlein, L. Letts] In immature or injured lungs, impaired alveolar gas exchange forces the use of elevated levels of inhaled oxygen to maintain life. But, at high concentrations oxygen induces lung injury, edema, and bronchopulmonary dysplasia, probably by stimulating the generation of reactive oxygen radicals and subsequent neutrophil infiltration. In addition to regulating neutrophil diapedesis, intercellular adhesion molecule-1 (ICAM-1) expression is marked on inflamed alveolar epithelium, suggesting a role for ICAM-1 in oxygen-induced, neutrophil-mediated parenchymal damage. To test this, we evaluated the rat anti-mouse ICAM-1 monoclonal antibody YN 1/1.7 in 2 protocols of oxygen-induced toxicity in adult, male Balb-c mice: ≥95% O2 for 84 hr and ≥95% O2 for 60 hr followed by 48 hr at 21% (ambient) O2. YN1/1.7 treatment partially attenuated the neutrophil infiltration, lung damage (lavage lactate dehydrogenase [LDH] activity) and dysfunction (reductions in respiratory system compliance [Crs] and diffusion capacity of the lungs for carbon monoxide [DLco] in the 84 hr exposure protocol. In the milder 60 hr exposure protocol, YN1/1.7 completely blocked the oxygen-induced lung dysfunction (reductions in Crs and DLco). These results confirm the contribution of leukocytes in the pathogenesis of pulmonary oxygen toxicity and indicate that antagonism of ICAM-1 may provide a therapeutic approach to reducing hyperoxic lung injury and dysfunction. Springer-Verlag New York Inc., 1992 Cell adhesion nationallicence Neutrophils nationallicence Acute lung injury nationallicence Alveolitis nationallicence Bronchopulmonary dysplasia nationallicence Pulmonary function nationallicence Wegner Craig Department of Pharmacology, Boehringer Ingelheim Pharmaceuticals, Inc., 90 East Ridge, P.O. Box 368, 06877, Ridgefield, CT, USA aut Wolyniec Walter Department of Pharmacology, Boehringer Ingelheim Pharmaceuticals, Inc., 90 East Ridge, P.O. Box 368, 06877, Ridgefield, CT, USA aut LaPlante April Department of Pharmacology, Boehringer Ingelheim Pharmaceuticals, Inc., 90 East Ridge, P.O. Box 368, 06877, Ridgefield, CT, USA aut Marschman Kristin Department of Pharmacology, Boehringer Ingelheim Pharmaceuticals, Inc., 90 East Ridge, P.O. Box 368, 06877, Ridgefield, CT, USA aut Lubbe Klaus Department of Molecular Biology, Boehringer Ingelheim Pharmaceuticals, Inc., 90 East Ridge, P.O. Box 368, 06877, Ridgefield, CT, USA aut Haynes Nancy Department of Immunology, Boehringer Ingelheim Pharmaceuticals, Inc., 90 East Ridge, P.O. Box 368, 06877, Ridgefield, CT, USA aut Rothlein Robert Department of Immunology, Boehringer Ingelheim Pharmaceuticals, Inc., 90 East Ridge, P.O. Box 368, 06877, Ridgefield, CT, USA aut Letts L. Department of Pharmacology, Boehringer Ingelheim Pharmaceuticals, Inc., 90 East Ridge, P.O. Box 368, 06877, Ridgefield, CT, USA aut Lung Springer-Verlag 170/5(1992-09-01), 267-279 0341-2040 170:5<267 1992 170 408 https://doi.org/10.1007/BF00566679 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00566679 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wegner Craig Department of Pharmacology, Boehringer Ingelheim Pharmaceuticals, Inc., 90 East Ridge, P.O. Box 368, 06877, Ridgefield, CT, USA aut NATIONALLICENCE 700 1- Wolyniec Walter Department of Pharmacology, Boehringer Ingelheim Pharmaceuticals, Inc., 90 East Ridge, P.O. Box 368, 06877, Ridgefield, CT, USA aut NATIONALLICENCE 700 1- LaPlante April Department of Pharmacology, Boehringer Ingelheim Pharmaceuticals, Inc., 90 East Ridge, P.O. Box 368, 06877, Ridgefield, CT, USA aut NATIONALLICENCE 700 1- Marschman Kristin Department of Pharmacology, Boehringer Ingelheim Pharmaceuticals, Inc., 90 East Ridge, P.O. Box 368, 06877, Ridgefield, CT, USA aut NATIONALLICENCE 700 1- Lubbe Klaus Department of Molecular Biology, Boehringer Ingelheim Pharmaceuticals, Inc., 90 East Ridge, P.O. Box 368, 06877, Ridgefield, CT, USA aut NATIONALLICENCE 700 1- Haynes Nancy Department of Immunology, Boehringer Ingelheim Pharmaceuticals, Inc., 90 East Ridge, P.O. Box 368, 06877, Ridgefield, CT, USA aut NATIONALLICENCE 700 1- Rothlein Robert Department of Immunology, Boehringer Ingelheim Pharmaceuticals, Inc., 90 East Ridge, P.O. Box 368, 06877, Ridgefield, CT, USA aut NATIONALLICENCE 700 1- Letts L. Department of Pharmacology, Boehringer Ingelheim Pharmaceuticals, Inc., 90 East Ridge, P.O. Box 368, 06877, Ridgefield, CT, USA aut NATIONALLICENCE 773 0- Lung Springer-Verlag 170/5(1992-09-01), 267-279 0341-2040 170:5<267 1992 170 408 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer