caa a22 4500 46904926X CHVBK 20180323132825.0 cr unu---uuuuu 170328e19920301xx s 000 0 eng 10.1007/BF00175978 doi (NATIONALLICENCE)springer-10.1007/BF00175978 The search for tumor-associated proteins in pleural effusions by means of monoclonal antibodies and a dot blot assay [Elektronische Daten] [Maria Lawniczak, Jan Sikora, Przemyslaw Kania, Jan Zeromski] Liquid moiety of 61 pleural effusions was tested for tumor-associated proteins (TAP) by means of a dot immunobinding (dot blot) assay (DIA) and a panel of monoclonal antibodies (Moabs). The sensitivity of the assay was checked using a purified, serially diluted carcinoembryonic antigen (CEA) preparation and an anti-CEA monoclonal IgG system. The latter was examined using both DIA and enzyme-linked immunosorbent assay ELISA solid phase assays in simulated conditions that mimicked the protein content of effusions. Finally, the results of DIA were compared to the immunohistochemistry carried out on cell sediments from the same effusions with similar Moabs. It was found that the prevalence of several TAPS, including CEA, epithelial membrane antigen (EMA), vimentin, tenascin, and Thomsen Friedenreich antigen, was significantly higher in the malignant effusions than in the nonmalignant ones. A total, larger than 2, of detected TAPS in a given fluid, was found almost exclusively in malignant effusions (p < 0.0001): The detection limit of the DIA for a CEA was determined at 5 ng/ml, while for the ELISA it was 1 ng/ml. Several TAPS, especially the CEA, could be detected in parallel tests, carried out on the liquid moiety and the cell sediments of malignant effusions. The evaluation of selected TAPS in pleural effusions by dot blot assay may be of clinical value. Springer-Verlag, 1992 Pleural effusions nationallicence Lung carcinoma nationallicence Monoclonal antibodies nationallicence Dot blot assay nationallicence Tumor associated proteins nationallicence Immunohistochemistry nationallicence Lawniczak Maria Department of Immunopathology, University Medical School, 49 Przybyszewskiego Street, Poznan, Poland aut Sikora Jan Department of Immunopathology, University Medical School, 49 Przybyszewskiego Street, Poznan, Poland aut Kania Przemyslaw Department of Immunopathology, University Medical School, 49 Przybyszewskiego Street, Poznan, Poland aut Zeromski Jan Department of Immunopathology, University Medical School, 49 Przybyszewskiego Street, Poznan, Poland aut Lung Springer-Verlag 170/2(1992-03-01), 65-74 0341-2040 170:2<65 1992 170 408 https://doi.org/10.1007/BF00175978 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00175978 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lawniczak Maria Department of Immunopathology, University Medical School, 49 Przybyszewskiego Street, Poznan, Poland aut NATIONALLICENCE 700 1- Sikora Jan Department of Immunopathology, University Medical School, 49 Przybyszewskiego Street, Poznan, Poland aut NATIONALLICENCE 700 1- Kania Przemyslaw Department of Immunopathology, University Medical School, 49 Przybyszewskiego Street, Poznan, Poland aut NATIONALLICENCE 700 1- Zeromski Jan Department of Immunopathology, University Medical School, 49 Przybyszewskiego Street, Poznan, Poland aut NATIONALLICENCE 773 0- Lung Springer-Verlag 170/2(1992-03-01), 65-74 0341-2040 170:2<65 1992 170 408 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer