caa a22 4500 469049359 CHVBK 20180323132826.0 cr unu---uuuuu 170328e19920301xx s 000 0 eng 10.1007/BF02921338 doi (NATIONALLICENCE)springer-10.1007/BF02921338 Sasano Hironobu Department of Pathology, Tohoku University School of Medicine, 2-1 Sciryo-machi Aoba-ku, Sen-dai-shi, 980, Miyagi-ken, Japan aut New approaches in human adrenocortical pathology: Assessment of adrenocortical function in surgical specimen of human adrenal glands [Elektronische Daten] [Hironobu Sasano] In adrenocortical pathology, it is important to localize the sites of steroid biosynthesis in order to obtain a better understanding of steroid metabolism. Previous morphological techniques, including light- and electron-microscopical examination, histochemistry, and immunohisto-chemistry of steroids, as well as biochemical studies, could not satisfactorily demonstrate which types of cortical cells produce which steroid hormones. Recent purification and characterization of specific steroidogenic enzymes, such as cytochrome P-450, that are involved in adrenal steroid biosynthesis have made it possible to generate specific antibodies and DNA probes against the enzymes that catalyze specific reaction(s) in the complicated biochemical cascade of steroidogenesis. By employing these antibodies and DNA probes, it has became possible to detect the expression of specific steroidogenic enzymes in surgically resected human adrenal cortex and its disorders. This can indicate the site(s) of specific steroid hormone(s) catalyzed by the expressed enzymes. Humana Press Inc., 1992 Endocrine Pathology Humana Press 3/1(1992-03-01), 4-13 1046-3976 3:1<4 1992 3 12022 https://doi.org/10.1007/BF02921338 text/html Onlinezugriff via DOI 1 review-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02921338 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Sasano Hironobu Department of Pathology, Tohoku University School of Medicine, 2-1 Sciryo-machi Aoba-ku, Sen-dai-shi, 980, Miyagi-ken, Japan aut NATIONALLICENCE 773 0- Endocrine Pathology Humana Press 3/1(1992-03-01), 4-13 1046-3976 3:1<4 1992 3 12022 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer