caa a22 4500 469049405 CHVBK 20180323132826.0 cr unu---uuuuu 170328e19920901xx s 000 0 eng 10.1007/BF02921355 doi (NATIONALLICENCE)springer-10.1007/BF02921355 Neural cell adhesion molecule (NCAM) in normal and neoplastic human pituitary tissues: Analysis by immunohistochemistry and in situ hybridization [Elektronische Daten] [Long Jin, Michael Stone, William Chandler, Ricardo Lloyd] The expression of the neural cell adhesion molecule (NCAM) in 6 normal human pituitaries and 25 pituitary adenomas was investigated by immunohistochemistry and in situ hybridization. NCAM protein and mRNA were present in all normal and neoplastic human pituitary tissues. There were tumor type-specific differences in the distribution of NCAM in various pituitary adenomas. Growth hormone adenomas and prolactin-producing adenomas usually expressed lower levels of NCAM, compared to null cell and gonadotroph adenomas. Adreno-corticotropic hormone adenomas expressed the highest levels of NCAM mRNA. Six freshly dissociated pituitary adenomas were cultured in serum-free medium for 7 days to analyze the regulation of NCAM mRNA by in situ hybridization. The lower levels of NCAM expression in growth hormone and prolactin adenomas were not present in cells cultured for 7 days in serum-free medium on extracellular matrix. Phorbol 12-myristate 13-acetate (PMA) stimulated NCAM mRNA expression in 5 of 6 tumors. Gonadotropin-releasing hormone and growth hormone-releasing hormone increased NCAM expression in some adenomas. This study demonstrates that there is a variable expression of NCAM in pituitary adenomas and that hypotha-lamic hormones and PMA can regulate NCAM mRNA levels in neoplastic pituitary cells. Humana Press Inc., 1992 Jin Long Department of Pathology, University of Michigan Medical Center, 1500 E. Medical Center Drive, Room 2G332, Box 0054, 48109-0054, Ann Arbor, MI aut Stone Michael Department of Pathology, University of Michigan Medical Center, 1500 E. Medical Center Drive, Room 2G332, Box 0054, 48109-0054, Ann Arbor, MI aut Chandler William Department of Pathology, University of Michigan Medical Center, 1500 E. Medical Center Drive, Room 2G332, Box 0054, 48109-0054, Ann Arbor, MI aut Lloyd Ricardo Department of Pathology, University of Michigan Medical Center, 1500 E. Medical Center Drive, Room 2G332, Box 0054, 48109-0054, Ann Arbor, MI aut Endocrine Pathology Humana Press 3/3(1992-09-01), 144-151 1046-3976 3:3<144 1992 3 12022 https://doi.org/10.1007/BF02921355 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02921355 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jin Long Department of Pathology, University of Michigan Medical Center, 1500 E. Medical Center Drive, Room 2G332, Box 0054, 48109-0054, Ann Arbor, MI aut NATIONALLICENCE 700 1- Stone Michael Department of Pathology, University of Michigan Medical Center, 1500 E. Medical Center Drive, Room 2G332, Box 0054, 48109-0054, Ann Arbor, MI aut NATIONALLICENCE 700 1- Chandler William Department of Pathology, University of Michigan Medical Center, 1500 E. Medical Center Drive, Room 2G332, Box 0054, 48109-0054, Ann Arbor, MI aut NATIONALLICENCE 700 1- Lloyd Ricardo Department of Pathology, University of Michigan Medical Center, 1500 E. Medical Center Drive, Room 2G332, Box 0054, 48109-0054, Ann Arbor, MI aut NATIONALLICENCE 773 0- Endocrine Pathology Humana Press 3/3(1992-09-01), 144-151 1046-3976 3:3<144 1992 3 12022 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer