caa a22 4500 469049413 CHVBK 20180323132826.0 cr unu---uuuuu 170328e19920901xx s 000 0 eng 10.1007/BF02921353 doi (NATIONALLICENCE)springer-10.1007/BF02921353 Immunohistochemical and immunoelectron microscopical localization of procollagen III peptide in papillary carcinoma of thyroid [Elektronische Daten] [Ryohei Katoh, Yoji Iida, Koichi Suzuki, Akira Kawaoi, Akira Muramatsu] The immunohistochemical localization of procollagen III peptide, a precursor of type III collagen, was examined in 38 papillary carcinomas and 25 follicular neoplasms using an immu-noperoxidase technique. Although localization of procollagen ill peptide was not demonstrated in normal follicular cells, distinct cytoplasmic immunostaining of neoplastic cells was frequently found in the tissues examined, as were stromal fibroblasts. Such cytoplasmic immunostaining was observed in 92.1 % of 38 papillary carcinomas and in 36.0% of 25 follicular neoplasms. Cytoplasmic immunoreactivity in papillary carcinomas was more intense at the peripheral zone of the tumor and correlated with the degree of invasiveness. The controls, in which the primary antibody was preabsorbed with procollagen ill peptide or replaced with normal rabbit serum, showed only faint background staining in all specimens. Immunoelectron microscopical examination revealed that electron-dense deposits, indicating procollagen III peptide, were located in the perinuclear space, Golgi apparatus, and rough endoplasmic reticulum of papillary carcinoma cells. These results suggest that neoplastic cells, especially papillary carcinoma cells, could play an important role in type III collagen production, possibly in connection with the creation of an environment that is conducive to their progression. Humana Press Inc., 1992 Katoh Ryohei Department of Pathology, Yamanashi Medical College School of Medicine, 1110 Shimokato, Tamaho-cho, Nakakoma-gun, 409-38, Yamanashi, Japan aut Iida Yoji Department of Pathology, Yamanashi Medical College School of Medicine, 1110 Shimokato, Tamaho-cho, Nakakoma-gun, 409-38, Yamanashi, Japan aut Suzuki Koichi Department of Pathology, Yamanashi Medical College School of Medicine, 1110 Shimokato, Tamaho-cho, Nakakoma-gun, 409-38, Yamanashi, Japan aut Kawaoi Akira Department of Pathology, Yamanashi Medical College School of Medicine, 1110 Shimokato, Tamaho-cho, Nakakoma-gun, 409-38, Yamanashi, Japan aut Muramatsu Akira Department of Surgery, Kofu City Hospital, Kofu, Japan aut Endocrine Pathology Humana Press 3/3(1992-09-01), 129-133 1046-3976 3:3<129 1992 3 12022 https://doi.org/10.1007/BF02921353 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02921353 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Katoh Ryohei Department of Pathology, Yamanashi Medical College School of Medicine, 1110 Shimokato, Tamaho-cho, Nakakoma-gun, 409-38, Yamanashi, Japan aut NATIONALLICENCE 700 1- Iida Yoji Department of Pathology, Yamanashi Medical College School of Medicine, 1110 Shimokato, Tamaho-cho, Nakakoma-gun, 409-38, Yamanashi, Japan aut NATIONALLICENCE 700 1- Suzuki Koichi Department of Pathology, Yamanashi Medical College School of Medicine, 1110 Shimokato, Tamaho-cho, Nakakoma-gun, 409-38, Yamanashi, Japan aut NATIONALLICENCE 700 1- Kawaoi Akira Department of Pathology, Yamanashi Medical College School of Medicine, 1110 Shimokato, Tamaho-cho, Nakakoma-gun, 409-38, Yamanashi, Japan aut NATIONALLICENCE 700 1- Muramatsu Akira Department of Surgery, Kofu City Hospital, Kofu, Japan aut NATIONALLICENCE 773 0- Endocrine Pathology Humana Press 3/3(1992-09-01), 129-133 1046-3976 3:3<129 1992 3 12022 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer