caa a22 4500 469049596 CHVBK 20180323132826.0 cr unu---uuuuu 170328e19920301xx s 000 0 eng 10.1007/BF02973980 doi (NATIONALLICENCE)springer-10.1007/BF02973980 Microbial transformation of aniline to acetaminophen [Elektronische Daten] [Sang Lee, Hyung Jin, Mi Son] In order to obtain acetaminophen, a popular analgesic-antipyretic, through microbial p-hydroxylation and N-acetylation of aniline, various fungi and bacteria were screened. Among them,Streptomyces species were chosen for strain improvement by the use of interspecific protoplast fusion technique. Two interspecific fused strains were developed betweenS. rimosus (N-cetylation function) andS. aureofaciens (p-hydroxylation function) and also betweenS. lividans andS. globisporus. For efficient protoplast fusion and cell wall regeneration, various conditions were examined. In a typical experiment of mixedS. rimosus (pro− his−) andS. aureofaciens (ilv−) protoplasts with 40% (w/v) polyethylene glycol 3350 (PEG) for 3 min gave 8.3×10−7 of fusion frequency. Treatment of mixedS. lividans (pant−) andS. globisporus (leu−) protoplasts with 50% (w/v) PEG for 3 min at 30°C gave 1.2×10−6 of frequency. Among the fused strains, up to 40-50% increase in p-hydroxylation power was observed. To investigate the possibility of plasmid involvement in p-hydroxylation of acetanilide, plasmid curing was attempted. We found that cells treated with acriflavine (at the frequency of 100%) and cells regenerated from protoplsts ofS. aureofaciens (2% frequency) lost their p-hydroxylation function. The Pharmaceutical Society of Korea, 1992 Protoplast fusion nationallicence plasmid curing nationallicence p-hydroxylation of aniline nationallicence Lee Sang College of Pharmacy, Seoul National University, Seoul, Korea aut Jin Hyung College of Pharmacy, Seoul National University, Seoul, Korea aut Son Mi College of Pharmacy, Seoul National University, Seoul, Korea aut Archives of Pharmacal Research Pharmaceutical Society of Korea 15/1(1992-03-01), 30-34 0253-6269 15:1<30 1992 15 12272 https://doi.org/10.1007/BF02973980 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02973980 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lee Sang College of Pharmacy, Seoul National University, Seoul, Korea aut NATIONALLICENCE 700 1- Jin Hyung College of Pharmacy, Seoul National University, Seoul, Korea aut NATIONALLICENCE 700 1- Son Mi College of Pharmacy, Seoul National University, Seoul, Korea aut NATIONALLICENCE 773 0- Archives of Pharmacal Research Pharmaceutical Society of Korea 15/1(1992-03-01), 30-34 0253-6269 15:1<30 1992 15 12272 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer