caa a22 4500 469049650 CHVBK 20180323132827.0 cr unu---uuuuu 170328e19920301xx s 000 0 eng 10.1007/BF02973981 doi (NATIONALLICENCE)springer-10.1007/BF02973981 Genetic analysis on bioconversion of aniline to acetaminophen in Streptomyces fradiae [Elektronische Daten] [Hyung Jin, Ae Park, Sang Lee] S. fradiae showed the highest acetanilide p-hydroxylation activity in the tested strains. AndS. fradiae was well characterized genetically, especially with respect to tylosin production. Two mutants, which lost hydroxylation, were isolated in 140 regenerated colonies from protoplasts. In restriction enzyme digesion of total DNAs, isolation of giant linear plasmid DNA and determination of antibiotic resistances to chloramphenicol, tylosin, hygromycin B and mitomycin C, any differences among mutants and a wild type strain were not detected. These facts suggest that lesion on 6,000 Kb chromosomal DNA was responsible for the lack of p-hydroxylation activity induced by protoplast formation and regeneration. The Pharmaceutical Society of Korea, 1992 Acetanilide p-hydroxylation nationallicence protoplast formation and regeneration nationallicence genetic instability nationallicence giant linear plasmid nationallicence Jin Hyung College of Pharmacy, Seoul National University, 151-742, Seoul, Korea aut Park Ae College of Pharmacy, Seoul National University, 151-742, Seoul, Korea aut Lee Sang College of Pharmacy, Seoul National University, 151-742, Seoul, Korea aut Archives of Pharmacal Research Pharmaceutical Society of Korea 15/1(1992-03-01), 35-40 0253-6269 15:1<35 1992 15 12272 https://doi.org/10.1007/BF02973981 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02973981 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jin Hyung College of Pharmacy, Seoul National University, 151-742, Seoul, Korea aut NATIONALLICENCE 700 1- Park Ae College of Pharmacy, Seoul National University, 151-742, Seoul, Korea aut NATIONALLICENCE 700 1- Lee Sang College of Pharmacy, Seoul National University, 151-742, Seoul, Korea aut NATIONALLICENCE 773 0- Archives of Pharmacal Research Pharmaceutical Society of Korea 15/1(1992-03-01), 35-40 0253-6269 15:1<35 1992 15 12272 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer