caa a22 4500 469049693 CHVBK 20180323132827.0 cr unu---uuuuu 170328e19920301xx s 000 0 eng 10.1007/BF02973983 doi (NATIONALLICENCE)springer-10.1007/BF02973983 Molecular cloning and M13 subcloning of genes encoding catechol dioxygenases [Elektronische Daten] [Youngsoo Kim, Bongsoo Choi, Kyung Min] Achromobacter xylosoxidans KF701 andPseudomonas putida (NAH7) were significantly different in degradative capability of aromatic compounds including benzoates. biphenyls, and naphthalene. However, both of the bacterial strains can grown on catechol as the sole carbon and energy source. Catechol 2,3-dioxygenase gene for naphthalene oxidation or biphenyl oxidation was cloned intoEscherichia coli HB101 from NAH7 megaplasmid ofP. putida or chromosomal DNA ofA. xylosoxidans KF701. AE. coli HB101 clone containing catechol 2,3-dioxygenase gene fromP. putida (NAH7) contains a recombinant plasmid with 3,6-kb pBR322 and 6-kb insert DNA. AnotherE. coli HB101 clone containing catechol 2,3-dioxygenase gene fromA. xylosoxidans KF701 has a recombinant plasmid with 4.4-kb pBR322 and 10-kb insert DNA. Physical maps of the recombinant plasmids were constructed, and catechol 2,3-dioxygenase gene in the recombinant plasmid was further localized and subcloned into M13. The cloned-catechol 2,3-dioxygenase gene products were identified as yellow bands on nondenaturaing polyacrylamide gel after electrophoresis followed by activity staining with catechol solution. The Pharmaceutical Society of Korea, 1992 Aromatic pollutant nationallicence naphthalene nationallicence biphenyl nationallicence catechol dioxygenase nationallicence gene cloning nationallicence Kim Youngsoo Department of Biochemistry, College of Pharmacy, Chungbuk National University, 360-763, Cheongju, Korea aut Choi Bongsoo Department of Biochemistry, College of Pharmacy, Chungbuk National University, 360-763, Cheongju, Korea aut Min Kyung Department of Biochemistry, College of Pharmacy, Chungbuk National University, 360-763, Cheongju, Korea aut Archives of Pharmacal Research Pharmaceutical Society of Korea 15/1(1992-03-01), 48-51 0253-6269 15:1<48 1992 15 12272 https://doi.org/10.1007/BF02973983 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02973983 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kim Youngsoo Department of Biochemistry, College of Pharmacy, Chungbuk National University, 360-763, Cheongju, Korea aut NATIONALLICENCE 700 1- Choi Bongsoo Department of Biochemistry, College of Pharmacy, Chungbuk National University, 360-763, Cheongju, Korea aut NATIONALLICENCE 700 1- Min Kyung Department of Biochemistry, College of Pharmacy, Chungbuk National University, 360-763, Cheongju, Korea aut NATIONALLICENCE 773 0- Archives of Pharmacal Research Pharmaceutical Society of Korea 15/1(1992-03-01), 48-51 0253-6269 15:1<48 1992 15 12272 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer