caa a22 4500 469049707 CHVBK 20180323132827.0 cr unu---uuuuu 170328e19920301xx s 000 0 eng 10.1007/BF02973985 doi (NATIONALLICENCE)springer-10.1007/BF02973985 A macrolide-lincosamide-streptogramin B resistance determinant gene ( ermJ ) cloned from B. anthracis 590 [Elektronische Daten] [Hee-Sun Kim, Eung-Chil Choi, Byong-Kak Kim, Young-In Park] Bacillus anthracis 590 having an inducible resistance determinant to MLS antibiotics was isolated from a soil sample in Korea. The resistance gene (ermJ was cloned by Southern blotting of chromosomal DNA fragment digested by various restriction enzymes and coloy hybridization method and the cloned plasmid was named as pBA423. The size of inserted DNA fragment to pBS42 vector was about 2.9 kb and the DNA sequence of the subcloned fragment (Hinc II-Hinc II, 1.4 kb) was determined. The DNA sequence ofermJ was composed of 357 bp for leader region and 861 bp for the structural gene. Because the leader sequence ofermJ was homologous to that ofermK, the expression ofermJ is also thought to be controlled by a transcriptionl attenuation mechanism. The Pharmaceutical Society of Korea, 1992 MLS antibiotics nationallicence inducible resistance nationallicence gene cloning nationallicence B. anthracis nationallicence Kim Hee-Sun College of Pharmacy, Seoul National University, 151-742, Seoul, Korea aut Choi Eung-Chil College of Pharmacy, Seoul National University, 151-742, Seoul, Korea aut Kim Byong-Kak College of Pharmacy, Seoul National University, 151-742, Seoul, Korea aut Park Young-In College of Pharmacy, Seoul National University, 151-742, Seoul, Korea aut Archives of Pharmacal Research Pharmaceutical Society of Korea 15/1(1992-03-01), 58-61 0253-6269 15:1<58 1992 15 12272 https://doi.org/10.1007/BF02973985 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02973985 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kim Hee-Sun College of Pharmacy, Seoul National University, 151-742, Seoul, Korea aut NATIONALLICENCE 700 1- Choi Eung-Chil College of Pharmacy, Seoul National University, 151-742, Seoul, Korea aut NATIONALLICENCE 700 1- Kim Byong-Kak College of Pharmacy, Seoul National University, 151-742, Seoul, Korea aut NATIONALLICENCE 700 1- Park Young-In College of Pharmacy, Seoul National University, 151-742, Seoul, Korea aut NATIONALLICENCE 773 0- Archives of Pharmacal Research Pharmaceutical Society of Korea 15/1(1992-03-01), 58-61 0253-6269 15:1<58 1992 15 12272 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer