caa a22 4500 46904974X CHVBK 20180323132827.0 cr unu---uuuuu 170328e19920301xx s 000 0 eng 10.1007/BF02973982 doi (NATIONALLICENCE)springer-10.1007/BF02973982 Bioconversion of aniline to acetaminophen and overproduction of acetaminophen by Streptomyces spp [Elektronische Daten] [Hyung Jin, Ae Park, Sang Lee] In order to obtain acetaminophen, a popular analgesic-antipyretic, though microbial p-hydroxylation and N-acetylation of aniline, variousStreptomyces strains were screened. Aniline N-acetylation activity was rather ubiquitous but p-hydroxylation activity was selective. Microbial conversion pathway of aniline to acetaminophen was considered to be through N-acetylation and p-hydroxylation orvice versa. However, depending on species used, o-hydroxylation and its degradation activity (S. fradiae) and acetaminophen degradation activity (S. coelicolar) were also detected. Among the screenedStreptomyces strains.S. fradiae NRRL 2702 showed the highest acetanilide p-hydroxylation activity (2-3% conversion rate). Furthermore, inS. fradiae carbon source and its concentration, phosphate ion concentration and pH of growth medium were found to play the crucial roles in p-hydroxylation activity. Through the proper combination of factors mentioned above, the ten times more activity (26-30% conversion rate) was attained. The Pharmaceutical Society of Korea, 1992 Aniline nationallicence acetaminophen nationallicence hydroxylation nationallicence Streptomyces fradiae nationallicence acetaminophen degradation activity nationallicence medium component effect nationallicence high production nationallicence Jin Hyung College of Pharmacy, Seoul National University, 151-742, Seoul, Korea aut Park Ae College of Pharmacy, Seoul National University, 151-742, Seoul, Korea aut Lee Sang College of Pharmacy, Seoul National University, 151-742, Seoul, Korea aut Archives of Pharmacal Research Pharmaceutical Society of Korea 15/1(1992-03-01), 41-47 0253-6269 15:1<41 1992 15 12272 https://doi.org/10.1007/BF02973982 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02973982 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jin Hyung College of Pharmacy, Seoul National University, 151-742, Seoul, Korea aut NATIONALLICENCE 700 1- Park Ae College of Pharmacy, Seoul National University, 151-742, Seoul, Korea aut NATIONALLICENCE 700 1- Lee Sang College of Pharmacy, Seoul National University, 151-742, Seoul, Korea aut NATIONALLICENCE 773 0- Archives of Pharmacal Research Pharmaceutical Society of Korea 15/1(1992-03-01), 41-47 0253-6269 15:1<41 1992 15 12272 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer