caa a22 4500 469049766 CHVBK 20180323132827.0 cr unu---uuuuu 170328e19920901xx s 000 0 eng 10.1007/BF02974057 doi (NATIONALLICENCE)springer-10.1007/BF02974057 Enzymatic study on acetanilide p -hydroxylase in Streptomyces fradiae [Elektronische Daten] [Hyung Jin, Ae Park, Sang Lee] S. fradiae exhibited the highest acetanilidep-hydroxylation activity among theStreptomyces spp. Studies with inhibitors (metyrapone, 2,6-dichloroindophenol, α,α′-dipyridyl,o-phenanthroline) and an absorption peak after CO treatment suggested thatS. fradiae hydroxylase activity was due to cytochrome p-450. This hydroxylase activity was increased to ten times in the cell extract containing 0.5 mM sodium azide. Further-more, the sedimentary activity in 105,000×g centrifugal forces and solubilization of the activity with Triton-X 100 implied that this enzyme was membrane bound monooxygenase. pH Optimum of the enzyme was 6.5 in membrane bound state. The Pharmaceutical Society of Korea, 1992 cytochrome P-450 nationallicence membrane bound enzyme nationallicence sodium azide for enzyme activation nationallicence optimum pH for acetailide p -hydroxylase nationallicence Jin Hyung College of Pharmacy, Seoul National University, 151-742, Seoul, Korea aut Park Ae College of Pharmacy, Seoul National University, 151-742, Seoul, Korea aut Lee Sang College of Pharmacy, Seoul National University, 151-742, Seoul, Korea aut Archives of Pharmacal Research Pharmaceutical Society of Korea 15/3(1992-09-01), 215-219 0253-6269 15:3<215 1992 15 12272 https://doi.org/10.1007/BF02974057 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02974057 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jin Hyung College of Pharmacy, Seoul National University, 151-742, Seoul, Korea aut NATIONALLICENCE 700 1- Park Ae College of Pharmacy, Seoul National University, 151-742, Seoul, Korea aut NATIONALLICENCE 700 1- Lee Sang College of Pharmacy, Seoul National University, 151-742, Seoul, Korea aut NATIONALLICENCE 773 0- Archives of Pharmacal Research Pharmaceutical Society of Korea 15/3(1992-09-01), 215-219 0253-6269 15:3<215 1992 15 12272 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer