caa a22 4500 469049820 CHVBK 20180323132827.0 cr unu---uuuuu 170328e19920901xx s 000 0 eng 10.1007/BF02974061 doi (NATIONALLICENCE)springer-10.1007/BF02974061 Biosynthesis of β-lactam antibiotics by cell-free extract from Lysobacter lactamgenus [Elektronische Daten] [Ju Roh, Doo Nam] Using cell-free extract ofLysobacter lactamgenus, enzymatic conversion of δ-l-(α-aminoadipyl)-l-cysteinyl-d-valine (ACV), the first substrate of β-lactam biosynthesis, into antibiotic compounds was attempted. In high performance liquid chromatographic (HPLC) analysis, the biosynthetic intermediates for cephalosporin antibiotics including isopenicillin N, deacetoxycephalosporin C, deacetylcephalosporin C and unknown cephem compound were detected in reaction mixtures. It implies that cephabacin compounds fromL. lactamgenus could be produced by biosynthetic routes through penicillin ring formation and its expansion to cephalosporin ring, likely as cephalosporin C fromCephalosporium or cephamycin C fromStreptomyces. Among biosynthetic enzyme in cell-free extract, the ring formation activity (isopenicillin N synthetase activity) was separated in 50-60% of ammonium sulfate fraction, and ring expansion activity (deacetoxycephalosporin C synthetase activity) was found to be in 40-50% fraction. The partially purified isopenicillin N synthetase could convert as much as 90% ACV to isopenicillin N during 6-hour reaction. The Pharmaceutical Society of Korea, 1992 β-lactam biosynthesis nationallicence Lysobacter lactamgenus nationallicence isopenicillin N synthetase nationallicence deacetoxycephalosporin C synthetase nationallicence Roh Ju College of Pharmacy, Yeungnam University, 712-749, Gyongsan, Korea aut Nam Doo College of Pharmacy, Yeungnam University, 712-749, Gyongsan, Korea aut Archives of Pharmacal Research Pharmaceutical Society of Korea 15/3(1992-09-01), 234-238 0253-6269 15:3<234 1992 15 12272 https://doi.org/10.1007/BF02974061 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02974061 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Roh Ju College of Pharmacy, Yeungnam University, 712-749, Gyongsan, Korea aut NATIONALLICENCE 700 1- Nam Doo College of Pharmacy, Yeungnam University, 712-749, Gyongsan, Korea aut NATIONALLICENCE 773 0- Archives of Pharmacal Research Pharmaceutical Society of Korea 15/3(1992-09-01), 234-238 0253-6269 15:3<234 1992 15 12272 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer