caa a22 4500 469049847 CHVBK 20180323132827.0 cr unu---uuuuu 170328e19920901xx s 000 0 eng 10.1007/BF02974053 doi (NATIONALLICENCE)springer-10.1007/BF02974053 Membrane-ordering effects of barbiturates on pure phospholipid model membranes [Elektronische Daten] [Jung Knag, Yong Chung, Goon Cho, Won Byun, Il Yun] Intramolecular excimer formation of 1,3-di(1-pyrenyl)propane (Py-3-Py) and fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene (DPH) were used to investigate the effects of barbiturates on the fluidity of model membranes of phosphatidylcholine (SPMVPC), phosphatidylserine (SPMVPS), and phosphatidylinositol (SPMVPI) fractions of synaptosomal plasma membrane vesicles (SPMV) isolated from bovine cerebral cortex. In a dose-dependent manner, barbiturates decreased the excimer to monomer fluorescence intensity ratio (I′/I) of Py-3-Py and increased the anisotropy(r), rotational relaxation time(φ), limiting anisotropy(r∞), and order parameter (S) of DPH in SPMVPC, SPMVPS and SPMVPI. This indicates that barbiturates decreased both the lateral and rotational diffusion of the probes in SPMVPC, SPMVPS and SPMVPI. The relative potencies of barbiturates in ordering the membranes were in the order: pentobarbital>hexobarbital>amobarbital>phenobarbital. This order correlates well with the anesthetic potencies of barbiturates and the potencies for enhancement of γ-aminobutyric acid-stimulated chloride uptake. Thus, it is strongly suggested that a close relationship might exist between the membrane-ordering effects of barbiturates and the chloride fluxes across SPMV. The Pharmaceutical Society of Korea, 1992 Barbiturates nationallicence membrane-ordering effects nationallicence fluorescent probe technique nationallicence Knag Jung College of Pharmacy, Kyung Sung University, 608-736, Pusan, Korea aut Chung Yong College of Pharmacy, Kyung Sung University, 608-736, Pusan, Korea aut Cho Goon College of Pharmacy, Kyung Sung University, 608-736, Pusan, Korea aut Byun Won College of Pharmacy, Kyung Sung University, 608-736, Pusan, Korea aut Yun Il College of Pharmacy, Kyung Sung University, 608-736, Pusan, Korea aut Archives of Pharmacal Research Pharmaceutical Society of Korea 15/3(1992-09-01), 196-203 0253-6269 15:3<196 1992 15 12272 https://doi.org/10.1007/BF02974053 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02974053 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Knag Jung College of Pharmacy, Kyung Sung University, 608-736, Pusan, Korea aut NATIONALLICENCE 700 1- Chung Yong College of Pharmacy, Kyung Sung University, 608-736, Pusan, Korea aut NATIONALLICENCE 700 1- Cho Goon College of Pharmacy, Kyung Sung University, 608-736, Pusan, Korea aut NATIONALLICENCE 700 1- Byun Won College of Pharmacy, Kyung Sung University, 608-736, Pusan, Korea aut NATIONALLICENCE 700 1- Yun Il College of Pharmacy, Kyung Sung University, 608-736, Pusan, Korea aut NATIONALLICENCE 773 0- Archives of Pharmacal Research Pharmaceutical Society of Korea 15/3(1992-09-01), 196-203 0253-6269 15:3<196 1992 15 12272 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer