caa a22 4500 469050136 CHVBK 20180323132828.0 cr unu---uuuuu 170328e19921201xx s 000 0 eng 10.1007/BF02974113 doi (NATIONALLICENCE)springer-10.1007/BF02974113 Cloning and overexpression of methylcatechol 2,3-dioxygenase gene from toluene-degrading Pseudomonas putida mt-2 (pWWO) [Elektronische Daten] [Jeongrai Lee, Kyung Min, Youngsoo Kim] Methylcatechol 2,3-dioxygenase encoded in pWWO megaplasmid ofPseudomonas putida mt-2 has been cloned and overexpressed inEscherichia coli. This enzyme gene has been localized inside 2.3-kbXhoI fragment derived from the pWWO megaplasmid. Analysis of enzyme activity and SDS-PAGE showed that the cloned methylcatechol 2,3-dioxygenase gene inE. coli was about 100 fold overexpressed compared with the parental gene inP. putida mt-2 (pWWO). The cloned enzyme exhibited higher ring-fission activity to catechol than catechol derivatives including 3-methylcatechol, 4-methylcatechol, and 4-chlorocatechol. The Pharmaceutical Society of Korea, 1992 Methylcatechol 2,3-dioxygenase nationallicence pWWO nationallicence gene cloning nationallicence overexpression nationallicence Lee Jeongrai Department of Biochemistry, College of Pharmacy, Chungbuk National University, 360-763, Cheongju, Korea aut Min Kyung Department of Biochemistry, College of Pharmacy, Chungbuk National University, 360-763, Cheongju, Korea aut Kim Youngsoo Department of Biochemistry, College of Pharmacy, Chungbuk National University, 360-763, Cheongju, Korea aut Archives of Pharmacal Research Pharmaceutical Society of Korea 15/4(1992-12-01), 360-364 0253-6269 15:4<360 1992 15 12272 https://doi.org/10.1007/BF02974113 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02974113 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lee Jeongrai Department of Biochemistry, College of Pharmacy, Chungbuk National University, 360-763, Cheongju, Korea aut NATIONALLICENCE 700 1- Min Kyung Department of Biochemistry, College of Pharmacy, Chungbuk National University, 360-763, Cheongju, Korea aut NATIONALLICENCE 700 1- Kim Youngsoo Department of Biochemistry, College of Pharmacy, Chungbuk National University, 360-763, Cheongju, Korea aut NATIONALLICENCE 773 0- Archives of Pharmacal Research Pharmaceutical Society of Korea 15/4(1992-12-01), 360-364 0253-6269 15:4<360 1992 15 12272 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer