caa a22 4500 469050144 CHVBK 20180323132828.0 cr unu---uuuuu 170328e19921201xx s 000 0 eng 10.1007/BF02974111 doi (NATIONALLICENCE)springer-10.1007/BF02974111 Conformation of group "a” epitope in hepatitis B surface antigen [Elektronische Daten] [Mun Chun, Won Park, Jin Bok, Ha Kim, Eung Choi, Byong Kim] To elucidate structure of group "a” epitope, mouse antibodies that express idiotype monoclonal antibody and anti-idiotype monoclonal antibody against the group-specific "a” determinant were purified by hydroxyapatite column. To obtain hepatitis B surface antigens (HBsAg), HBsAg positive blood was sequencially purified by ammonium sulfate precipitation, hydroxyapatite, sepharose 4B column chromatography and ultracentrifugation. The major protein (p25) and glycoprotein (gp30) of HBsAg were, isolated by concanavalin-A sepharose 4B. The ability of p25-gp30 among the HBsAg to inhibit the idiotype-anti-idiotype reaction was dependent on conformation, since reduced and alkylated p25-gp30 virtually lost their inhibitory capacity when compared to native HBsAg. The data suggest that hepatitis B antigen is a conformational antigen critically dependent upon the disulfide bonds of p25-pg30. The Pharmaceutical Society of Korea, 1992 idiotype antibody nationallicence anti-idiotype antibody nationallicence HBsAg nationallicence epitope nationallicence competitive ELISA inhibition nationallicence Chun Mun Department of Microbial Chemistry, College of Pharmacy, Seoul National University, 151-742, Seoul, Korea aut Park Won Seoul Women's University, 139-242, Seoul, Korea aut Bok Jin Department of Microbial Chemistry, College of Pharmacy, Seoul National University, 151-742, Seoul, Korea aut Kim Ha Department of Microbial Chemistry, College of Pharmacy, Seoul National University, 151-742, Seoul, Korea aut Choi Eung Department of Microbial Chemistry, College of Pharmacy, Seoul National University, 151-742, Seoul, Korea aut Kim Byong Department of Microbial Chemistry, College of Pharmacy, Seoul National University, 151-742, Seoul, Korea aut Archives of Pharmacal Research Pharmaceutical Society of Korea 15/4(1992-12-01), 347-355 0253-6269 15:4<347 1992 15 12272 https://doi.org/10.1007/BF02974111 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02974111 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chun Mun Department of Microbial Chemistry, College of Pharmacy, Seoul National University, 151-742, Seoul, Korea aut NATIONALLICENCE 700 1- Park Won Seoul Women's University, 139-242, Seoul, Korea aut NATIONALLICENCE 700 1- Bok Jin Department of Microbial Chemistry, College of Pharmacy, Seoul National University, 151-742, Seoul, Korea aut NATIONALLICENCE 700 1- Kim Ha Department of Microbial Chemistry, College of Pharmacy, Seoul National University, 151-742, Seoul, Korea aut NATIONALLICENCE 700 1- Choi Eung Department of Microbial Chemistry, College of Pharmacy, Seoul National University, 151-742, Seoul, Korea aut NATIONALLICENCE 700 1- Kim Byong Department of Microbial Chemistry, College of Pharmacy, Seoul National University, 151-742, Seoul, Korea aut NATIONALLICENCE 773 0- Archives of Pharmacal Research Pharmaceutical Society of Korea 15/4(1992-12-01), 347-355 0253-6269 15:4<347 1992 15 12272 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer