caa a22 4500 469050195 CHVBK 20180323132828.0 cr unu---uuuuu 170328e19920601xx s 000 0 eng 10.1007/BF02974092 doi (NATIONALLICENCE)springer-10.1007/BF02974092 Transbllayer effects of n-alkanols on the fluidity of phospholipid model membranes [Elektronische Daten] [Il Yun, Jung-Sook Kang] Selective quenching of 1,6-diphenyl-1,3,5-hexatriene (DPH) by trinitrophenyl groups was utilized to examine the transbilayer fluidity asymmetry of model membranes of phospholipids (SPMVPL) extracted from synaptosomal plasma membrane vesicles (SPMV). The polarization (P), anisotropy (r), limiting anisotropy (r∞), and order parameter (S) of DPH in the inner monolayer were 0.019, 0.014, 0.018, and 0.047, respectively, greater than calculated for the outer monolayer of SPMVPL. Selective quenching of DPH by trinitrophenyl groups was also utilized to examine the effects of n-alkanols on the individual monolayer structure of SPMVPL. n-Alkanols fluidized the hydrocarbon region of bulk SPMVPL and the potencies of n-alkanols up to 1-nonanol increased with carbon chain length. It appears that the potencies in bilayer fluidization increase by 1 order of magnitude as the carbon chain length increases by two carbon atoms. The cut-off phenomenon was reached at 1-decanol, where further increase in hydrocarbon length resulted in a decrease in pharmacological activity. The n-alkanols had greater fluidizing effects on the outer monolayer as compared to the inner monolayer of SPMVPL, even though these selective effects tended to become weaker as the carbon chain length increased. Thus, it has been proven that n-alkanols exhibit selective rather than nonselective fluidizing effects within transbilayer domains of SPMVPL. The Pharmaceutical Society of Korea, 1992 n-Alkanols nationallicence transbilayer fluidity asymmetry nationallicence phospholipid model membranes nationallicence fluorescent probe technique nationallicence Yun Il Department of Dental Pharmacology and Biophysics, Pusan national University, 602-739, Pusan, Korea aut Kang Jung-Sook Department of Oral Biochemistry and Molecular Biology College of Dentistry, Pusan National University, 602-739, Pusan, Korea aut Archives of Pharmacal Research Pharmaceutical Society of Korea 15/2(1992-06-01), 152-161 0253-6269 15:2<152 1992 15 12272 https://doi.org/10.1007/BF02974092 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02974092 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yun Il Department of Dental Pharmacology and Biophysics, Pusan national University, 602-739, Pusan, Korea aut NATIONALLICENCE 700 1- Kang Jung-Sook Department of Oral Biochemistry and Molecular Biology College of Dentistry, Pusan National University, 602-739, Pusan, Korea aut NATIONALLICENCE 773 0- Archives of Pharmacal Research Pharmaceutical Society of Korea 15/2(1992-06-01), 152-161 0253-6269 15:2<152 1992 15 12272 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer