caa a22 4500 46905025X CHVBK 20180323132828.0 cr unu---uuuuu 170328e19920601xx s 000 0 eng 10.1007/BF02974085 doi (NATIONALLICENCE)springer-10.1007/BF02974085 Influence of TMB-8 on secretion of catecholamines from the perfused rat adrenal glands [Elektronische Daten] [Dong-Yoon Lim, Chong-Dae Kim, Gi-Wan Ahn] An attempt was made to investigate the effect of TMB-8 [3,4,5-trimethoxybenzoate-8 (N,N-diethylamino) octyl ester], which is known to be an inhibitor of intracellular Ca2+ release, on catecholamines (CA) secretion evoked by Ach, excess K+, DMPP, McN-A-343 and caffeine from the isolated perfused rat adrenal glands and to clearify its mechanism of action. The pretreatment with a low dose of TMB-8 (10 μM) for 20 min led to marked inhibition in CA secretion evoked by Ach (5.32 mM), excess K+ (56 mM), DMPP (100 μM), McN-A-343 (100 μM) and BAY-K 8644 (10−5M). Caffeine-induced CA secretion was similar to that of control only during the first periods (0-3 min) but thereafter marked inhibition in CA secretion evoked by caffeine was observed during the rest periods up to 30 min. The increased moderate concentration of TMB-8 (30 μM) caused the result similar to that of 10 μM TMB-8. However, in adrenal glands preloaded with a high dose of TMB-8 (100 μM), CA releases evoked by Ach, excess K+, DMPP, McN-A-343 and caffeine were almost completely blocked by the drug. These experimental data demonstrate that TMB-8 may inhibit cholinergic receptor-mediated and also depolarization-dependent CA secretion, suggesting that these TMB-8 effects seem to be mediated through inhibiting influx of extracellular calcium into the rat adrenal medullary chromaffin cells as well as reducing the release of calcium from intracellular sources. The Pharmaceutical Society of Korea, 1992 TMB-8 nationallicence catecholamines nationallicence adrenal gland nationallicence intracellular calcium nationallicence Lim Dong-Yoon College of Medicine, Chosun University, 501-759, Kwang Ju, Korea aut Kim Chong-Dae College of Medicine, Chosun University, 501-759, Kwang Ju, Korea aut Ahn Gi-Wan College of Medicine, Chosun University, 501-759, Kwang Ju, Korea aut Archives of Pharmacal Research Pharmaceutical Society of Korea 15/2(1992-06-01), 115-125 0253-6269 15:2<115 1992 15 12272 https://doi.org/10.1007/BF02974085 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02974085 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lim Dong-Yoon College of Medicine, Chosun University, 501-759, Kwang Ju, Korea aut NATIONALLICENCE 700 1- Kim Chong-Dae College of Medicine, Chosun University, 501-759, Kwang Ju, Korea aut NATIONALLICENCE 700 1- Ahn Gi-Wan College of Medicine, Chosun University, 501-759, Kwang Ju, Korea aut NATIONALLICENCE 773 0- Archives of Pharmacal Research Pharmaceutical Society of Korea 15/2(1992-06-01), 115-125 0253-6269 15:2<115 1992 15 12272 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer