caa a22 4500 46905509X CHVBK 20180323132841.0 cr unu---uuuuu 170328e19920101xx s 000 0 eng 10.1007/BF00376439 doi (NATIONALLICENCE)springer-10.1007/BF00376439 Carnosine and anserine concentrations in the quadriceps femoris muscle of healthy humans [Elektronische Daten] [A. Mannion, P. Jakeman, M. Dunnett, R. Harris, P. Willan] Summary: The content of anserine and carnosine in the lateral portion of the quadriceps femoris muscle of 50 healthy, human subjects has been studied. Anserine was undetectable in all muscle samples examined. Muscle carnosine values for the group conformed to a normal distribution with a mean (SD) value of 20.0 (4.7) mmol · kg−1 of dry muscle mass. The concentration of carnosine was significantly higher in the muscle of male subjects (21.3, 4.2 mmol · kg−1 dry mass) than in females of a similar age and training status (17.5, 4.8 mmol · kg−1 dry mass) (P< 0.005). The test-retest reliability of measures was determined on a subgroup of 17 subjects. No significant difference in mean carnosine concentration was found between the two trials [21.5 (4.0) and 22.0 (5.2) mmol · kg−1 dry muscle mass; P>0.05]. The importance of carnosine as a physicochemical buffer within human muscle was examined by calculating its buffering ability over the physiological pH range. From the range of carnosine concentrations observed (7.2-30.7 mmol · kg−1 dry muscle mass), it was estimated that the dipeptide could buffer between 2.4 and 10.1 mmol H+ · kg−1 dry mass over the physiological pH range 7.1-6.5, contributing, on average, approximately 7% to the total muscle buffering. This suggests that in humans, in contrast to many other species, carnosine is of only limited importance in preventing the reduction in pH observed during high intensity exercise. Springer-Verlag, 1992 Carnosine nationallicence Anserine nationallicence Skeletal muscle buffering nationallicence Mannion A. School of Sport and Exercise Sciences, University of Birmingham, B15 2TT, UK aut Jakeman P. School of Sport and Exercise Sciences, University of Birmingham, B15 2TT, UK aut Dunnett M. Department of Comparative Physiology, Animal Health Trust, Newmarket, UK aut Harris R. Department of Comparative Physiology, Animal Health Trust, Newmarket, UK aut Willan P. Department of Cell and Structural Biology, University of Manchester, UK aut European Journal of Applied Physiology and Occupational Physiology Springer-Verlag 64/1(1992-01-01), 47-50 0301-5548 64:1<47 1992 64 421 https://doi.org/10.1007/BF00376439 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00376439 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mannion A. School of Sport and Exercise Sciences, University of Birmingham, B15 2TT, UK aut NATIONALLICENCE 700 1- Jakeman P. School of Sport and Exercise Sciences, University of Birmingham, B15 2TT, UK aut NATIONALLICENCE 700 1- Dunnett M. Department of Comparative Physiology, Animal Health Trust, Newmarket, UK aut NATIONALLICENCE 700 1- Harris R. Department of Comparative Physiology, Animal Health Trust, Newmarket, UK aut NATIONALLICENCE 700 1- Willan P. Department of Cell and Structural Biology, University of Manchester, UK aut NATIONALLICENCE 773 0- European Journal of Applied Physiology and Occupational Physiology Springer-Verlag 64/1(1992-01-01), 47-50 0301-5548 64:1<47 1992 64 421 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer