caa a22 4500 469055529 CHVBK 20180323132842.0 cr unu---uuuuu 170328e19920701xx s 000 0 eng 10.1007/BF00636218 doi (NATIONALLICENCE)springer-10.1007/BF00636218 Changes in blood cell response following strenuous physical exercise [Elektronische Daten] [H. Kvernmo, J. Olsen, B. Østerud] Summary: The generation of tumour necrosis factor (TNF) and tissue factor activity in lipopolysaccharide (LPS) stimulated blood were studied in 25 healthy subjects before and after physical exercise of different intensities. Of the subjects a group of 9 were athletes who trained once to twice every day of the week, a second group of 8 exercised 3-7 times a week, and a third group of 8 exercised 4-5 times a month. The production of TNF in freshly drawn LPS stimulated blood in heparin, drawn from top athletes at rest was significantly lower than in the other subjects. The LPS induced concentrations of TNF-α of 2.73 (SEM 1.05) ng · ml−1 in the blood of the top athletes compared to 5.08 (SEM 0.7) ng · ml−1 and 7.6 (SEM 1.6) ng · ml−1, respectively, in the other two groups. The group that trained the least had the highest values. Immediately after exercise, the monocytes appeared to be less responsive to LPS stimulation, as a reduction of 47%-48% was observed in the top athletes and in the other group of well-trained individuals. The group that trained the least, which was also subjected to the least stressful exercise, had a 33% reduction in TNF production. Within 6 h the TNF concentration was back to pre-exercise values. Within 6 h the TNF concentration was back to pre-exercise values. In contrast to TNF production, the LPS induced tissue factor activity of monocytes was significantly higher in the top athletes and in the group of well-trained individuals when measured immediately after 10 min of exercise compared to pre-exercise values. The increase in tissue factor activity was probably a reflection of simultaneous granulocyte- and platelet activation which is known to enhance LPS induced tissue factor activity in monocytes. Except for the period immediately after exercise, there was a significant correlation between LPS induced TNF and tissue factor activity in the whole blood of the participants. In conclusion, hard physical training appeared to suppress the availability of TNF in stimulated blood. This was even more pronounced immediately after strenuous physical exercise. Springer-Verlag, 1992 Monocytes nationallicence Tumour necrosis factor nationallicence Tissue factor nationallicence Physical training nationallicence Kvernmo H. Institute of Medical Biology, University of Tromsø, 9000, Tromsø, Norway aut Olsen J. Institute of Medical Biology, University of Tromsø, 9000, Tromsø, Norway aut Østerud B. Institute of Medical Biology, University of Tromsø, 9000, Tromsø, Norway aut European Journal of Applied Physiology and Occupational Physiology Springer-Verlag 64/4(1992-07-01), 318-322 0301-5548 64:4<318 1992 64 421 https://doi.org/10.1007/BF00636218 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00636218 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kvernmo H. Institute of Medical Biology, University of Tromsø, 9000, Tromsø, Norway aut NATIONALLICENCE 700 1- Olsen J. Institute of Medical Biology, University of Tromsø, 9000, Tromsø, Norway aut NATIONALLICENCE 700 1- Østerud B. Institute of Medical Biology, University of Tromsø, 9000, Tromsø, Norway aut NATIONALLICENCE 773 0- European Journal of Applied Physiology and Occupational Physiology Springer-Verlag 64/4(1992-07-01), 318-322 0301-5548 64:4<318 1992 64 421 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer