caa a22 4500 469056789 CHVBK 20180323132846.0 cr unu---uuuuu 170328e19920301xx s 000 0 ita 10.1007/BF03002966 doi (NATIONALLICENCE)springer-10.1007/BF03002966 Ruffo A. Laboratorio di Biochimica Dipartimento di Chimica delle Sostanze Naturali, Università degli Studi di Napoli, 49 -80131, Napoli aut Interferenza del siero sulla determinazione enzimatica di gliossi-lato in alcuni terreni di cultura. Nota di Michela Festa e Antonella Angiolillo [Elektronische Daten] [A. Ruffo] Interference from serum on the enzymatic determination of glyoxylate in celles grown on media containing pyruvate It has been investigated on the rate of the decrement of the absorbance in the UV of NADH, which occurs during the enzymatic analysis of glyoxylate in the supernatants of cells grown on D-MEM and 10% foetal bovine serum. It was found that the extra-NADH oxidized during the incubation was due to the effect of the lactic dehydrogenase present in the serum able to reduce the pyruvate contained in D-MEM into equivalents of lactic acid. Therefore the coincidence of sera together with pyruvate in a culture medium must be taken in account to avoid erroneous analytical results about the concentration of metabolites determined in the supernatants by methods based on the measure of the enzymatic decrement of the absorbance of NADH in the UV. Accademia nazionale dei Lincei, 1992 Supernatant of cell cultures nationallicence Determination of glyoxylate nationallicence Decarboxylation of oxalomalate nationallicence Rendiconti Lincei Springer-Verlag 3/1(1992-03-01), 75-80 1120-6349 3:1<75 1992 3 12210 https://doi.org/10.1007/BF03002966 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF03002966 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Ruffo A. Laboratorio di Biochimica Dipartimento di Chimica delle Sostanze Naturali, Università degli Studi di Napoli, 49 -80131, Napoli aut NATIONALLICENCE 773 0- Rendiconti Lincei Springer-Verlag 3/1(1992-03-01), 75-80 1120-6349 3:1<75 1992 3 12210 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer