caa a22 4500 469060336 CHVBK 20180323132855.0 cr unu---uuuuu 170328e19921201xx s 000 0 eng 10.1007/BF01193809 doi (NATIONALLICENCE)springer-10.1007/BF01193809 Functional morphology of Gené's organ in Haemaphysalis longicornis (Acari: Ixodidae) [Elektronische Daten] [H. Kakuda, T. Mōri, S. Shiraishi] The structure and function of Gené's organ, which secretes the egg wax, were investigated inHaemaphysalis longicornis. The hatching rates of eggs deposited before and after the prevention of the eversion of Gené's organ were compared at the conditions of 30°C, 100% RH and soaking in liquid paraffin. The movement of Gené's organ was observed, and the structures of this organ in unfed and ovipositing females were examined with both light and scanning electron microscopes. The hatching rates of eggs without contact with Gené's organ were markedly lower (4.9%) than that of normal eggs (94.5%) even at the suitable condition for eggs (30°C, 100% RH). In contrast, the hatching rates of eggs soaked in liquid paraffin without contact of Gené's organ were considerably higher (71.8%) than that of eggs without both Gené's organ contact and soaking in liquid paraffin. When oviposition commenced, the camerostome of the female was widened by a downswing of the capitulum, the paired horns of Gené's organ emerged through it, and the oviposited egg from the genital aperture was coated with egg wax. At the same time of retraction of the horns which grip an egg, the capitulum was swung up and the egg was loaded on the scutum. Gené's organ included the outer and inner cuticles, and the outer one is highly folded like bellows when the horns of this organ were retracted into the haemocoel. The undeveloped glands lying in the peripheral region of Gené's organ in the unfed stage commenced their enlargement at the time of the onset of feeding, and they became large tubular glands in the 1-day ovipositing female. The columnar glandular cells of the tubular glands extended their domed apical region into the lumen and contained numerous secretory granules during oviposition. These results suggest that the tubular glands secrete the egg wax which is indispensable for egg survival by serving as a waterproofing agent. Elsevier Science Publishers B. V, 1993 Kakuda H. Zoological Laboratory, Faculty of Agriculture, Kyushu University, 46-06, Fukuoka 812, Japan aut Mōri T. Zoological Laboratory, Faculty of Agriculture, Kyushu University, 46-06, Fukuoka 812, Japan aut Shiraishi S. Zoological Laboratory, Faculty of Agriculture, Kyushu University, 46-06, Fukuoka 812, Japan aut Experimental & Applied Acarology Kluwer Academic Publishers 16/3(1992-12-01), 263-275 0168-8162 16:3<263 1992 16 10493 https://doi.org/10.1007/BF01193809 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01193809 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kakuda H. Zoological Laboratory, Faculty of Agriculture, Kyushu University, 46-06, Fukuoka 812, Japan aut NATIONALLICENCE 700 1- Mōri T. Zoological Laboratory, Faculty of Agriculture, Kyushu University, 46-06, Fukuoka 812, Japan aut NATIONALLICENCE 700 1- Shiraishi S. Zoological Laboratory, Faculty of Agriculture, Kyushu University, 46-06, Fukuoka 812, Japan aut NATIONALLICENCE 773 0- Experimental & Applied Acarology Kluwer Academic Publishers 16/3(1992-12-01), 263-275 0168-8162 16:3<263 1992 16 10493 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer