caa a22 4500 469060565 CHVBK 20180323132855.0 cr unu---uuuuu 170328e19921101xx s 000 0 eng 10.1007/BF01201498 doi (NATIONALLICENCE)springer-10.1007/BF01201498 Molecular polymorphisms of house dust mite allergens [Elektronische Daten] [W. Thomas, K. Chua, W. Smith] Previous studies from this laboratory have described the primary amino acid sequences of the group I and group II allergens fromDermatophagoides pteronyssinus andD. farinae. This report concentrates on polymorphisms of allergens within a species. Firstly, four cDNA clones ofDer fII produced by polymerase chain reaction have been sequenced and are compared to the sequences published previously by ourselves and others. Although the sequences come from different sources, Australia and Japan, the overriding conclusion is one of similarity, with only two possible non-conservative changes in the six sequences. The nucleotides were also very conserved including the 3′ untranslated regions, although some non-coding differences could be found which may provide a genetic marker. Experiments are reported to help define the group IIID. pteronyssinus allergens. Previous studies have characterised the group III ofD. farinae as a Mr 29-kDa molecule which can be defined by monoclonal antibodies. A Mr 17-kDa molecule ofD. pteronyssinus has been reported with an almost identical N-terminal sequence. Here it is described thatDer fIII isolated from different preparations of spent mite media by affinity chromatography have predominantly Mr 32-, 28- and 21-kDa forms which vary in degree from batch to batch. 83% of adults and 38% of children react with the preparation by radioimmune dot-blot. The difference between the children and adults is statistically significant and reactivity can be to at least the 32- and 28-kDa form. Antisera produced in mice against theDer fIII react toD. pteronyssinus mite extract by Western blotting primarily to a 32-kDa moiety, but also 28- and 21-kDa forms in some extracts. Elsevier Science Publishers B.V., 1992 Thomas W. Division of Molecular Biology, Western Australian Research Institute for Child Health, Princess Margaret Hospital, Box D184, GPO, 6001, Perth, W.A., Australia aut Chua K. Division of Molecular Biology, Western Australian Research Institute for Child Health, Princess Margaret Hospital, Box D184, GPO, 6001, Perth, W.A., Australia aut Smith W. Division of Molecular Biology, Western Australian Research Institute for Child Health, Princess Margaret Hospital, Box D184, GPO, 6001, Perth, W.A., Australia aut Experimental & Applied Acarology Kluwer Academic Publishers 16/1-2(1992-11-01), 153-164 0168-8162 16:1-2<153 1992 16 10493 https://doi.org/10.1007/BF01201498 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01201498 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Thomas W. Division of Molecular Biology, Western Australian Research Institute for Child Health, Princess Margaret Hospital, Box D184, GPO, 6001, Perth, W.A., Australia aut NATIONALLICENCE 700 1- Chua K. Division of Molecular Biology, Western Australian Research Institute for Child Health, Princess Margaret Hospital, Box D184, GPO, 6001, Perth, W.A., Australia aut NATIONALLICENCE 700 1- Smith W. Division of Molecular Biology, Western Australian Research Institute for Child Health, Princess Margaret Hospital, Box D184, GPO, 6001, Perth, W.A., Australia aut NATIONALLICENCE 773 0- Experimental & Applied Acarology Kluwer Academic Publishers 16/1-2(1992-11-01), 153-164 0168-8162 16:1-2<153 1992 16 10493 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer