caa a22 4500 469060581 CHVBK 20180323132856.0 cr unu---uuuuu 170328e19921101xx s 000 0 eng 10.1007/BF01201499 doi (NATIONALLICENCE)springer-10.1007/BF01201499 A comparative study of allergenic and potentially allergenic enzymes from Dermatophagoides pteronyssinus, D. farinae and Euroglyphus maynei [Elektronische Daten] [Geoffrey Stewart, Catherine Bird, Karen Krska, Matthew Colloff, Philip Thompson] The presence of the enzymatically active allergens equivalent toDer p I (cysteine protease),Der p III (serine protease) and amylase in extracts ofDermatophagoides pteronyssinus, D. farinae andEuroglyphus maynei was determined using appropriate enzymatic techniques. Biochemical equivalents of all three allergens were present in each extract studied. Studies also showed that the mite extracts contained a variety of other biochemically active enzymes including trypsin, chymotrypsin, carboxypeptidase A and B, glucoamylase and lysozyme. Marked differences in the relative concentrations of some of these enzymes in different mite extracts were observed, particularly trypsin and carboxypeptidase A. The enzymes were physicochemically similar to equivalent enzymes from vertebrate and invertebrate sources. Chromatofocusing studies of faecal extracts derived fromD. pteronyssinus andD. farinae showed that several isoforms of each enzyme were present. The data indicated that there were more trypsin isoforms, with pI over a wider range, in extracts prepared fromD. pteronyssinus. Proteases and carbohydrases were also found in extracts prepared from faecally enriched material suggesting that they were endoperitrophic and associated with mite digestion. The data suggest that not only are the group I, III and amylase allergens a consistent feature of most pyroglyphid dust mites but also that other proteases and carbohydrases present in mite faeces are allergenic. Elsevier Science Publishers B.V., 1992 Stewart Geoffrey The Western Australian Research Institute for Child Health, Princess Margaret Hospital, Thomas St., Subiaco, 6008, Perth, W.A., Australia aut Bird Catherine The Western Australian Research Institute for Child Health, Princess Margaret Hospital, Thomas St., Subiaco, 6008, Perth, W.A., Australia aut Krska Karen The Western Australian Research Institute for Child Health, Princess Margaret Hospital, Thomas St., Subiaco, 6008, Perth, W.A., Australia aut Colloff Matthew Dept. of Zoology, University of Glasgow, Glasgow, UK aut Thompson Philip Dept. of Medicine, University of Western Australia, Nedlands, Perth, W.A., Australia aut Experimental & Applied Acarology Kluwer Academic Publishers 16/1-2(1992-11-01), 165-180 0168-8162 16:1-2<165 1992 16 10493 https://doi.org/10.1007/BF01201499 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01201499 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Stewart Geoffrey The Western Australian Research Institute for Child Health, Princess Margaret Hospital, Thomas St., Subiaco, 6008, Perth, W.A., Australia aut NATIONALLICENCE 700 1- Bird Catherine The Western Australian Research Institute for Child Health, Princess Margaret Hospital, Thomas St., Subiaco, 6008, Perth, W.A., Australia aut NATIONALLICENCE 700 1- Krska Karen The Western Australian Research Institute for Child Health, Princess Margaret Hospital, Thomas St., Subiaco, 6008, Perth, W.A., Australia aut NATIONALLICENCE 700 1- Colloff Matthew Dept. of Zoology, University of Glasgow, Glasgow, UK aut NATIONALLICENCE 700 1- Thompson Philip Dept. of Medicine, University of Western Australia, Nedlands, Perth, W.A., Australia aut NATIONALLICENCE 773 0- Experimental & Applied Acarology Kluwer Academic Publishers 16/1-2(1992-11-01), 165-180 0168-8162 16:1-2<165 1992 16 10493 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer