caa a22 4500 469060611 CHVBK 20180323132856.0 cr unu---uuuuu 170328e19920201xx s 000 0 eng 10.1007/BF01194936 doi (NATIONALLICENCE)springer-10.1007/BF01194936 Histochemical changes in the midgut of two ixodid tick species Boophilus microplus and Rhipicephalus appendiculatus during digestion of the blood meal [Elektronische Daten] [A. Agyei, N. Runham, N. Blackstock] The changes in the midgut epithelia of two ixodid tick species,Boophilus microplus andRhipicephalus appendiculatus, have been studied using several histochemical techniques. It was revealed that there is an accumulation of RNA at the time of tick attachment to the host and prior to the arrival of the blood meal, indicating that the midgut digest cell is furnished with the machinery characteristic of a synthetic cell. There appears to be a synchrony in the appearance of granules with peroxidase activity and the uptake of haemoglobin into the midgut digest cells. Alkaline phosphatase activity was observed in the midgut epithelia of all ticks except in a few of the long-starved ticks, and was concentrated in the apical plasma membrane regions of those digest cells involved in absorption and the intracellular digestion of haemoglobin. The presence of these enzymes suggests that the midgut digest cell is a multifunctional cell capable of both secretory and digestive activities. The colloidal material in the midgut lumen was found to result from the accretion of several products both secreted and excreted by the midgut epithelial cells and exhibited different staining reactions depending on which component dominated. The nature of the material suggests that in addition to its digestive function it may serve as a sink to bind all the by-products of digestion and thereby facilitate their excretion. Elsevier Science Publishers B.V., 1992 Agyei A. School of Biological Sciences, Univ. Col. of North Wales, LL57 2UW, Bangor, Gwynedd, UK aut Runham N. School of Biological Sciences, Univ. Col. of North Wales, LL57 2UW, Bangor, Gwynedd, UK aut Blackstock N. School of Biological Sciences, Univ. Col. of North Wales, LL57 2UW, Bangor, Gwynedd, UK aut Experimental & Applied Acarology Kluwer Academic Publishers 13/3(1992-02-01), 187-212 0168-8162 13:3<187 1992 13 10493 https://doi.org/10.1007/BF01194936 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01194936 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Agyei A. School of Biological Sciences, Univ. Col. of North Wales, LL57 2UW, Bangor, Gwynedd, UK aut NATIONALLICENCE 700 1- Runham N. School of Biological Sciences, Univ. Col. of North Wales, LL57 2UW, Bangor, Gwynedd, UK aut NATIONALLICENCE 700 1- Blackstock N. School of Biological Sciences, Univ. Col. of North Wales, LL57 2UW, Bangor, Gwynedd, UK aut NATIONALLICENCE 773 0- Experimental & Applied Acarology Kluwer Academic Publishers 13/3(1992-02-01), 187-212 0168-8162 13:3<187 1992 13 10493 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer