caa a22 4500 469060743 CHVBK 20180323132856.0 cr unu---uuuuu 170328e19920301xx s 000 0 eng 10.1007/BF01195081 doi (NATIONALLICENCE)springer-10.1007/BF01195081 Saliva-activated transmission (SAT) of Thogoto virus: dynamics of SAT factor activity in the salivary glands of Rhipicephalus appendiculatus, Amblyomma variegatum , and Boophilus microplus ticks [Elektronische Daten] [L. Jones, M. Matthewson, P. Nuttall] Thogoto (THO) virus is transmitted from infected to uninfected ticks when co-feeding on uninfected guinea-pigs, even though the guinea-pigs do not develop a detectable viraemia. This form of non-viraemic transmission is potentiated by a factor (s) secreted by the saliva of ticks and hence has been termed saliva-activated transmission (SAT). The synthesis of the SAT factor by the salivary glands of three ixodid tick species was determined by placing uninfected nymphal ticks on guineapigs that were subsequently inoculated with a mixture of THO virus and salivary gland extract (SGE) derived from one of the tick species. SAT factor activity was measured by determining the number of nymphs that acquired THO virus. For the three-host ixodid species,Rhipicephalus appendiculatus andAmblyomma variegatum, maximum enhancement of THO virus transmission was observed when salivary glands were derived from uninfected, female ticks that had fed for a period of 6 or 8 days, respectively. In contrast, when salivary glands were derived form uninfected femaleBoophilus microplus, a one-host ixodid tick species, enhancement of THO virus transmission was observed throughout the tick feeding period. Thus, the natural feeding behaviour of ticks appears to be an important factor in determining the relative importance of these vectors in mediating SAT. Elsevier Science Publishers B.V., 1992 Jones L. Institute of Virology and Environmental Microbiology, Natural Environmental Research Council, Mansfield Road, OX1 3SR, Oxford, UK aut Matthewson M. Pitman-Moore Ltd, Berkhamsted Hill, Berkhamsted, Herts, UK aut Nuttall P. Institute of Virology and Environmental Microbiology, Natural Environmental Research Council, Mansfield Road, OX1 3SR, Oxford, UK aut Experimental & Applied Acarology Kluwer Academic Publishers 13/4(1992-03-01), 241-248 0168-8162 13:4<241 1992 13 10493 https://doi.org/10.1007/BF01195081 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01195081 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jones L. Institute of Virology and Environmental Microbiology, Natural Environmental Research Council, Mansfield Road, OX1 3SR, Oxford, UK aut NATIONALLICENCE 700 1- Matthewson M. Pitman-Moore Ltd, Berkhamsted Hill, Berkhamsted, Herts, UK aut NATIONALLICENCE 700 1- Nuttall P. Institute of Virology and Environmental Microbiology, Natural Environmental Research Council, Mansfield Road, OX1 3SR, Oxford, UK aut NATIONALLICENCE 773 0- Experimental & Applied Acarology Kluwer Academic Publishers 13/4(1992-03-01), 241-248 0168-8162 13:4<241 1992 13 10493 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer