caa a22 4500 469060999 CHVBK 20180323132857.0 cr unu---uuuuu 170328e19920501xx s 000 0 eng 10.1007/BF01219106 doi (NATIONALLICENCE)springer-10.1007/BF01219106 Booth T. NERC Institute of Virology and Environmental Microbiology, Mansfield Road, OX1 3SR, Oxford, UK aut Observation on the composition and biosynthesis of egg wax lipids in the cattle tick, Boophilus microplus [Elektronische Daten] [T. Booth] The biosynthesis of wax lipids by Gené's organ, the egg waxing organ in ticks, was investigated. Gené's organ, a complex dermal gland system, applies a superficial wax layer to the eggs during oviposition which prevents desiccation and is essential for egg viability. The detailed anatomy and histology of the three gland cell types are unambiguously described. Serial sectioning of ticks showed that all three gland cell types are capable of contributing to the egg wax. The wax synthetic ability of these three gland types was characterized. The composition of wax lipids extracted from the surface egg wax, and from the three types of wax gland dissected from ovipositing ticks, was analysed using thin-layer and gas-liquid chromatography. Injection of ovipositing ticks with radiolabelled acetate resulted in the incorporation of the label into wax lipids by gland cells of Gené's organ. The egg wax was a complex mixture of long-chain alkanes and fatty acid esters. The gland cells contained a greater proportion of shorter chain alkanes than were present in the egg surface wax. Some unsaturated long-chain fatty acids were present, and these were more abundant in the gland cells than in the surface wax of oviposited eggs, suggesting that oxidation occurs after oviposition. The results confirm that the tubular glands, acinar accessory glands and lobular glands of Gené's organ all contribute to the egg waxes, although the lipid components differed in relative abundance. The results are also consistent with alkane synthesis from fatty acids in Gené's organ by a chain-elongation-decarboxcylation pathway. Elsevier Science Publishers B.V., 1992 Experimental & Applied Acarology Kluwer Academic Publishers 14/2(1992-05-01), 137-149 0168-8162 14:2<137 1992 14 10493 https://doi.org/10.1007/BF01219106 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01219106 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Booth T. NERC Institute of Virology and Environmental Microbiology, Mansfield Road, OX1 3SR, Oxford, UK aut NATIONALLICENCE 773 0- Experimental & Applied Acarology Kluwer Academic Publishers 14/2(1992-05-01), 137-149 0168-8162 14:2<137 1992 14 10493 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer