caa a22 4500 46906126X CHVBK 20180323132858.0 cr unu---uuuuu 170328e19921001xx s 000 0 eng 10.1007/BF01246563 doi (NATIONALLICENCE)springer-10.1007/BF01246563 Molecular approach in spider mites (Acari: Tetranychidae): Preliminary data on ribosomal DNA sequences [Elektronische Daten] [M. Navajas, D. Cotton, S. Kreiter, J. Gutierrez] DNA sequence data were used to examine phylogenetic relationships between six species of economically important Tetranychidae mites:Eotetranychus carpini (Oudemans),E. pruni (Reck),Tetranychus pacificus McGregor,T. mcdanieli McGregor,T. turkestani Ugarov & Nikolski andT. urticae Koch. With primers directed toward conserved elements flanking the target region, the Polymerase Chain Reaction was used to amplify the ITS2 spacer of the ribosomal DNA molecule. The nucleotide sequence of a 300-bp fragment of the ITS2 was determined by direct sequencing and nucleotide divergence used for intra-generic comparison in mites. The resulting phylogenetic tree expressing interspecific relationships in genusTetranychus agrees with morphological data. The study demonstrates the usefulness of the approach in the assessment of the systematics and evolution of the group. Elsevier Science Publishers B. V, 1992 Navajas M. Laboratoire d'Acarologie, INRA-ENSA.M-ORSTOM, 2 Place P. Viala, 34060, Montpellier, France aut Cotton D. Laboratoire d'Acarologie, INRA-ENSA.M-ORSTOM, 2 Place P. Viala, 34060, Montpellier, France aut Kreiter S. Laboratoire d'Acarologie, INRA-ENSA.M-ORSTOM, 2 Place P. Viala, 34060, Montpellier, France aut Gutierrez J. Laboratoire d'Acarologie, INRA-ENSA.M-ORSTOM, 2 Place P. Viala, 34060, Montpellier, France aut Experimental & Applied Acarology Kluwer Academic Publishers 15/4(1992-10-01), 211-218 0168-8162 15:4<211 1992 15 10493 https://doi.org/10.1007/BF01246563 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01246563 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Navajas M. Laboratoire d'Acarologie, INRA-ENSA.M-ORSTOM, 2 Place P. Viala, 34060, Montpellier, France aut NATIONALLICENCE 700 1- Cotton D. Laboratoire d'Acarologie, INRA-ENSA.M-ORSTOM, 2 Place P. Viala, 34060, Montpellier, France aut NATIONALLICENCE 700 1- Kreiter S. Laboratoire d'Acarologie, INRA-ENSA.M-ORSTOM, 2 Place P. Viala, 34060, Montpellier, France aut NATIONALLICENCE 700 1- Gutierrez J. Laboratoire d'Acarologie, INRA-ENSA.M-ORSTOM, 2 Place P. Viala, 34060, Montpellier, France aut NATIONALLICENCE 773 0- Experimental & Applied Acarology Kluwer Academic Publishers 15/4(1992-10-01), 211-218 0168-8162 15:4<211 1992 15 10493 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer