caa a22 4500 469061383 CHVBK 20180323132858.0 cr unu---uuuuu 170328e19920901xx s 000 0 eng 10.1007/BF00302872 doi (NATIONALLICENCE)springer-10.1007/BF00302872 A second ferritin L subunit is encoded by an intronless gene in the mouse [Elektronische Daten] [Francoise Renaudie, Abdel-Kader Yachou, Bernard Grandchamp, Richard Jones, Carole Beaumont] Multiple homologous sequences for the ferritin L subunit are present in mammalian genomes, but so far, only one expressed gene has been described. Here we report the isolation of a cDNA from a mouse bone marrow library, corresponding to an isoform of the mouse ferritin L subunit. This new subunit, that we named Lg, differs from the L subunit of ten amino acids. Specific amplification of mouse genomic DNA using the polymerase chain reaction (PCR) confirmed the presence of this Lg sequence in the mouse genome but also suggested that it must be encoded by an intronless gene. Using a series of different Lg-specific oligonucleotides as probes, we subsequently isolated a genomic clone containing an uninterrupted sequence, identical to the Lg cDNA. This Lg gene lacks introns and does not contain the 28 base pairs (bp) conserved motif usually present at the 5′ end of most ferritin mRNAs, which confers translational regulation by iron. When transiently transfected into K562 cells, this Lg genomic clone is actively transcribed, suggesting that, although it possesses the characteristics of a processed pseudogene, it is likely to correspond to the gene encoding this new ferritin subunit. Springer-Verlag New York Inc, 1992 Renaudie Francoise Laboratoire de Genetique Moleculaire, Faculte X. Bichat, 75018, Paris, France aut Yachou Abdel-Kader Laboratoire de Genetique Moleculaire, Faculte X. Bichat, 75018, Paris, France aut Grandchamp Bernard Laboratoire de Genetique Moleculaire, Faculte X. Bichat, 75018, Paris, France aut Jones Richard Institute of Molecular Medicine, John Radcliffe Hospital, Headington, OX3 9DU, Oxford, UK aut Beaumont Carole Laboratoire de Genetique Moleculaire, Faculte X. Bichat, 75018, Paris, France aut Mammalian Genome Springer-Verlag 2/3(1992-09-01), 143-149 0938-8990 2:3<143 1992 2 335 https://doi.org/10.1007/BF00302872 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00302872 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Renaudie Francoise Laboratoire de Genetique Moleculaire, Faculte X. Bichat, 75018, Paris, France aut NATIONALLICENCE 700 1- Yachou Abdel-Kader Laboratoire de Genetique Moleculaire, Faculte X. Bichat, 75018, Paris, France aut NATIONALLICENCE 700 1- Grandchamp Bernard Laboratoire de Genetique Moleculaire, Faculte X. Bichat, 75018, Paris, France aut NATIONALLICENCE 700 1- Jones Richard Institute of Molecular Medicine, John Radcliffe Hospital, Headington, OX3 9DU, Oxford, UK aut NATIONALLICENCE 700 1- Beaumont Carole Laboratoire de Genetique Moleculaire, Faculte X. Bichat, 75018, Paris, France aut NATIONALLICENCE 773 0- Mammalian Genome Springer-Verlag 2/3(1992-09-01), 143-149 0938-8990 2:3<143 1992 2 335 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer