caa a22 4500 469061413 CHVBK 20180323132858.0 cr unu---uuuuu 170328e19921201xx s 000 0 eng 10.1007/BF00355432 doi (NATIONALLICENCE)springer-10.1007/BF00355432 The genes for the inter-α-inhibitor family share a homologous organization in human and mouse [Elektronische Daten] [Jean Salier, Vera Verga, Janine Doly, Maryam Diarra-Mehropour, Robert Erickson] Inter-α-inhibitor (IαI) and related molecules in human are comprised of three evolutionarily related, heavy (H) chains and one light (L) chain, also termed bikunin. The latter originates from a precursor molecule that is cleaved to yield the bikunin and another protein designated α-1-microglobulin (A1m). The four H and L chains are encoded by four distinct genes designated H1, H2, H3, and L. The L and H2 genes are localized onto human chromosomes (chr) 9 and 10, respectively, whereas the H1 and H3 genes are tandemly arranged on chr 3. Mouse poly(A)+ RNAs or endonuclease-restricted mouse DNA were analyzed by standard and pulsedfield gel electrophoresis (PFGE) techniques in agarose gels and blot-hybridized with human H1, H2, H3 or L cDNA probes. The variable sized transcripts and unique restriction fragment patterns detected with each probe indicate that four genes, including one common L gene for Alm and bikunin also exist in mouse. The co-migration of H1- and H3-hybridizing fragments on PFGE suggest that the mouse H1 and H3 genes are also tandemly arranged. An Msp I restriction fragment length polymorphism (RFLP) in the mouse L gene (proposed symbol, Intin-4) links this gene to other genes already mapped at mouse Chr 4 near the brown (b) locus, a homologous region to the human chr 9q32-34 band where the human IαI L gene is located. Therefore, a similar number and arrangement of IαI genes is found in mouse and human, including the triplication of an H gene ancestor. These results point to an ancient origin of this complex set of genes. Springer-Verlag New York Inc, 1992 Salier Jean Institut National de la Santé et de la Recherche Médicale, Unit-78, 76230, Boisguillaume, France aut Verga Vera Department of Human Genetics, University of Michigan Medical School, 48109-0618, Ann Arbor, Michigan, USA aut Doly Janine Institut de Recherches Scientifiques sur le Cancer, CNRS UPR-37, 94800, Villejuif, France aut Diarra-Mehropour Maryam Institut National de la Santé et de la Recherche Médicale, Unit-295, 76800, Saint-Etienne-du-Rouvray, France aut Erickson Robert Department of Human Genetics, University of Michigan Medical School, 48109-0618, Ann Arbor, Michigan, USA aut Mammalian Genome Springer-Verlag 2/4(1992-12-01), 233-239 0938-8990 2:4<233 1992 2 335 https://doi.org/10.1007/BF00355432 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00355432 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Salier Jean Institut National de la Santé et de la Recherche Médicale, Unit-78, 76230, Boisguillaume, France aut NATIONALLICENCE 700 1- Verga Vera Department of Human Genetics, University of Michigan Medical School, 48109-0618, Ann Arbor, Michigan, USA aut NATIONALLICENCE 700 1- Doly Janine Institut de Recherches Scientifiques sur le Cancer, CNRS UPR-37, 94800, Villejuif, France aut NATIONALLICENCE 700 1- Diarra-Mehropour Maryam Institut National de la Santé et de la Recherche Médicale, Unit-295, 76800, Saint-Etienne-du-Rouvray, France aut NATIONALLICENCE 700 1- Erickson Robert Department of Human Genetics, University of Michigan Medical School, 48109-0618, Ann Arbor, Michigan, USA aut NATIONALLICENCE 773 0- Mammalian Genome Springer-Verlag 2/4(1992-12-01), 233-239 0938-8990 2:4<233 1992 2 335 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer