caa a22 4500 469061537 CHVBK 20180323132858.0 cr unu---uuuuu 170328e19920601xx s 000 0 eng 10.1007/BF00353858 doi (NATIONALLICENCE)springer-10.1007/BF00353858 Abbott Cathy Department of Genetics and Biometry, University College London, Wolfson House, 4 Stephenson Way, NW1 2HE, London, UK aut Characterization of mouse-hamster somatic cell hybrids by PCR: A panel of mouse-specific primers for each chromosome [Elektronische Daten] [Cathy Abbott] Mouse/hamster somatic cell hybrids form a valuable resource for mouse gene mapping. Characterization of these hybrids by isozyme analysis can be technically demanding and time-consuming. Species-specific polymerase chain reaction (PCR), where a mouse gene but not its homolog in the hamster is amplified, can provide an alternative means of characterization. Mouse-specific primers have been designed for at least one gene on each of the mouse autosomes and the X Chromosome (Chr). Primers are chosen to correspond to untranslated regions of the mouse gene concerned, in order to decrease the chance of crosshybridization with the homologous hamster gene. These primer sequences are presented, together with the conditions for their use. Springer-Verlag New York Inc, 1992 Mammalian Genome Springer-Verlag 2/2(1992-06-01), 106-109 0938-8990 2:2<106 1992 2 335 https://doi.org/10.1007/BF00353858 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00353858 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Abbott Cathy Department of Genetics and Biometry, University College London, Wolfson House, 4 Stephenson Way, NW1 2HE, London, UK aut NATIONALLICENCE 773 0- Mammalian Genome Springer-Verlag 2/2(1992-06-01), 106-109 0938-8990 2:2<106 1992 2 335 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer