caa a22 4500 46906157X CHVBK 20180323132859.0 cr unu---uuuuu 170328e19920601xx s 000 0 eng 10.1007/BF00353856 doi (NATIONALLICENCE)springer-10.1007/BF00353856 The mink gene for the λ light immunoglobulin chain: Characterization of cDNA and chromosomal localization [Elektronische Daten] [T. Khlebodarova, N. Matveeva, O. Serov, A. Najakshin, E. Belousov, S. Bogachev, O. Baranov] A cDNA library from mink spleen was constructed by use of the phage λgt11. The library was screened using polyvalent serum raised against the mink immunoglobulin λ chain. As a result, several clones expressing mink immunoglobulin λ light chains were identified. Sequencing of one of the clones with an 803 bp insert was performed. The insert comprised nearly the entire coding region for the mature λ light immunoglobulin gene with the exception of the leader polypeptide and several amino acids of the RF1 region of the V segment. Compared with the rabbit, mouse and human λ light immunoglobulin genes, the homology of the cloned sequence was found to be highest relative to the rabbit gene. With the cloned mink cDNA containing the C-region only as a probe, the DNAs from mink-Chinese hamster hybrid clones were studied. The results of segregation analysis of this mink cDNA sequence and mink chromosomes in the mink-Chinese hamster clone panel allowed us to assign the gene for the λ light immunoglobulin constant polypeptide (IGLC) to mink Chromosome (Chr) 4. Springer-Verlag New York Inc, 1992 Khlebodarova T. Institute of Cytology and Genetics, Academy of Sciences of the USSR, Siberian Department, Novosibirsk-90, USSR aut Matveeva N. Institute of Cytology and Genetics, Academy of Sciences of the USSR, Siberian Department, Novosibirsk-90, USSR aut Serov O. Institute of Cytology and Genetics, Academy of Sciences of the USSR, Siberian Department, Novosibirsk-90, USSR aut Najakshin A. Institute of Cytology and Genetics, Academy of Sciences of the USSR, Siberian Department, Novosibirsk-90, USSR aut Belousov E. Institute of Cytology and Genetics, Academy of Sciences of the USSR, Siberian Department, Novosibirsk-90, USSR aut Bogachev S. Institute of Cytology and Genetics, Academy of Sciences of the USSR, Siberian Department, Novosibirsk-90, USSR aut Baranov O. Institute of Cytology and Genetics, Academy of Sciences of the USSR, Siberian Department, Novosibirsk-90, USSR aut Mammalian Genome Springer-Verlag 2/2(1992-06-01), 96-99 0938-8990 2:2<96 1992 2 335 https://doi.org/10.1007/BF00353856 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00353856 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Khlebodarova T. Institute of Cytology and Genetics, Academy of Sciences of the USSR, Siberian Department, Novosibirsk-90, USSR aut NATIONALLICENCE 700 1- Matveeva N. Institute of Cytology and Genetics, Academy of Sciences of the USSR, Siberian Department, Novosibirsk-90, USSR aut NATIONALLICENCE 700 1- Serov O. Institute of Cytology and Genetics, Academy of Sciences of the USSR, Siberian Department, Novosibirsk-90, USSR aut NATIONALLICENCE 700 1- Najakshin A. Institute of Cytology and Genetics, Academy of Sciences of the USSR, Siberian Department, Novosibirsk-90, USSR aut NATIONALLICENCE 700 1- Belousov E. Institute of Cytology and Genetics, Academy of Sciences of the USSR, Siberian Department, Novosibirsk-90, USSR aut NATIONALLICENCE 700 1- Bogachev S. Institute of Cytology and Genetics, Academy of Sciences of the USSR, Siberian Department, Novosibirsk-90, USSR aut NATIONALLICENCE 700 1- Baranov O. Institute of Cytology and Genetics, Academy of Sciences of the USSR, Siberian Department, Novosibirsk-90, USSR aut NATIONALLICENCE 773 0- Mammalian Genome Springer-Verlag 2/2(1992-06-01), 96-99 0938-8990 2:2<96 1992 2 335 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer