caa a22 4500 469061596 CHVBK 20180323132859.0 cr unu---uuuuu 170328e19920101xx s 000 0 eng 10.1007/BF00355835 doi (NATIONALLICENCE)springer-10.1007/BF00355835 Molecular organization and evolution of the D17Leh80 -like loci in the mouse t complex [Elektronische Daten] [Valery Filippov, Elena Fedorova, Igor Rogozin, Nikolai Kholodilov, Anatoly Ruvinsky] We determined the sequence of Tu80, one of the molecular clones derived from microdissected fragments of Chromosome (Chr) 17. The sequence data demonstrated that Tu80 contains an open reading frame (ORF) of 204 bp. Two sequences within the ORF, one homologous to the LINE1 element and the other to the first intron of the C ε gene of mouse immunoglobulin, were observed. A separate sequence, homologous to Tu80, designated as NOV1, was isolated from a genomic library of mouse Chr 17. NOV1 was found to contain an inserted B2 repeat, making it structurally different from Tu80. The sequences of Tu80 and NOV1 were compared with those of LINE1 and the first intron of the C ε gene. The results suggest that the ancestor of the Tu80-like sequence might have arisen through recombination between a LINE1 element and the C ε gene. It is concluded that Tu80 and NOV1 might have resulted from duplication of an ancestral sequence followed by divergence. The comparative analysis also demonstrated a high degree of conservation of the LINE1-like sequence in Tu80 and NOV1. Based on the structure of human, rat, rabbit, and mouse LINE1 fragments, as well as those of NOV1 and Tu80, a phylogenetic tree was constructed. The available data tend to support the assumption that the ancestor for the Tu80-like sequence might have arisen not later than 27-33 million years ago. Springer-Verlag New York Inc, 1992 Filippov Valery Institute of Cytology and Genetics, Siberian Branch of the Academy of Sciences of the USSR, Novosibirsk, Russia aut Fedorova Elena Institute of Cytology and Genetics, Siberian Branch of the Academy of Sciences of the USSR, Novosibirsk, Russia aut Rogozin Igor Institute of Cytology and Genetics, Siberian Branch of the Academy of Sciences of the USSR, Novosibirsk, Russia aut Kholodilov Nikolai Institute of Cytology and Genetics, Siberian Branch of the Academy of Sciences of the USSR, Novosibirsk, Russia aut Ruvinsky Anatoly Institute of Cytology and Genetics, Siberian Branch of the Academy of Sciences of the USSR, Novosibirsk, Russia aut Mammalian Genome Springer-Verlag 3/1(1992-01-01), 11-16 0938-8990 3:1<11 1992 3 335 https://doi.org/10.1007/BF00355835 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00355835 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Filippov Valery Institute of Cytology and Genetics, Siberian Branch of the Academy of Sciences of the USSR, Novosibirsk, Russia aut NATIONALLICENCE 700 1- Fedorova Elena Institute of Cytology and Genetics, Siberian Branch of the Academy of Sciences of the USSR, Novosibirsk, Russia aut NATIONALLICENCE 700 1- Rogozin Igor Institute of Cytology and Genetics, Siberian Branch of the Academy of Sciences of the USSR, Novosibirsk, Russia aut NATIONALLICENCE 700 1- Kholodilov Nikolai Institute of Cytology and Genetics, Siberian Branch of the Academy of Sciences of the USSR, Novosibirsk, Russia aut NATIONALLICENCE 700 1- Ruvinsky Anatoly Institute of Cytology and Genetics, Siberian Branch of the Academy of Sciences of the USSR, Novosibirsk, Russia aut NATIONALLICENCE 773 0- Mammalian Genome Springer-Verlag 3/1(1992-01-01), 11-16 0938-8990 3:1<11 1992 3 335 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer