caa a22 4500 469061901 CHVBK 20180323132900.0 cr unu---uuuuu 170328e19920301xx s 000 0 eng 10.1007/BF00352460 doi (NATIONALLICENCE)springer-10.1007/BF00352460 Mapping and expression of the ubiquitin-activating enzyme E1 ( Ube1 ) gene in the mouse [Elektronische Daten] [Christine Disteche, Eldad Zacksenhaus, David Adler, Steven Bressler, Bernadette Keitz, Verne Chapman] The nucleotide sequence of the human cDNA encoding ubiquitin-activating enzyme E1 is more than 99% identical with the human A1S9T cDNA, a gene that has been shown to complement the temperature-sensitive mutant mouse cell line, tsA1S9. The amino acid sequences of the proteins encoded by these two cDNA sequences are identical, and both cDNAs were previously shown to be located in the same region of the human X chromosome; thus, ubiquitin-activating enzyme E1 and A1S9T appear to be the same gene, designated UBE1. By in situ hybridization to metaphase chromosomes from male mice and by Southern blot analysis of male and female mouse DNA, we show that, in the mouse, a human UBE1 cDNA probe identified both X- and Y-linked loci. Ube1 is located at band A2 of the mouse X Chromosome (Chr) and Ube2 on the short arm of the Y Chr. This is in contrast to the situation in the human, where there is no evidence for Y-linked sequences related to UBE1. Mapping of the Ube1 gene in interspecific backcrosses between Mus spretus and C57BL/6 shows that the Ube1 locus maps close to Timp, in a conserved region of the mouse and human X Chrs that include Otc, Cybb, Syn1, Timp, and Araf. Expression of Ube1 on the inactive X Chr was examined to determine whether this gene is subject to X-Chr inactivation in the mouse, as there is previous evidence that the human UBE1 gene escapes, at least partially, X inactivation. Sequencing of reverse transcriptase polymerase chain reaction (RT-PCR) products from M. spretus, C57BL/6J, and T(X;16)16H x M. spretus F1 female mice indicates that the mouse Ube1 gene is subject to X-Chr inactivation in vivo. This represents a new example of differences between the sex chromosomes of mouse and human. Springer-Verlag New York Inc, 1992 Disteche Christine Department of Pathology SM-30, University of Washington, 98195, Seattle, Washington, USA aut Zacksenhaus Eldad Division of Immunology and Cancer, Hospital for Sick Children, M5G 1X8, Toronto, Ontario, Canada aut Adler David Department of Pathology SM-30, University of Washington, 98195, Seattle, Washington, USA aut Bressler Steven Department of Pathology SM-30, University of Washington, 98195, Seattle, Washington, USA aut Keitz Bernadette Molecular Biology Department, Roswell Park Memorial Institute, 14263, Buffalo, New York, USA aut Chapman Verne Molecular Biology Department, Roswell Park Memorial Institute, 14263, Buffalo, New York, USA aut Mammalian Genome Springer-Verlag 3/3(1992-03-01), 156-161 0938-8990 3:3<156 1992 3 335 https://doi.org/10.1007/BF00352460 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00352460 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Disteche Christine Department of Pathology SM-30, University of Washington, 98195, Seattle, Washington, USA aut NATIONALLICENCE 700 1- Zacksenhaus Eldad Division of Immunology and Cancer, Hospital for Sick Children, M5G 1X8, Toronto, Ontario, Canada aut NATIONALLICENCE 700 1- Adler David Department of Pathology SM-30, University of Washington, 98195, Seattle, Washington, USA aut NATIONALLICENCE 700 1- Bressler Steven Department of Pathology SM-30, University of Washington, 98195, Seattle, Washington, USA aut NATIONALLICENCE 700 1- Keitz Bernadette Molecular Biology Department, Roswell Park Memorial Institute, 14263, Buffalo, New York, USA aut NATIONALLICENCE 700 1- Chapman Verne Molecular Biology Department, Roswell Park Memorial Institute, 14263, Buffalo, New York, USA aut NATIONALLICENCE 773 0- Mammalian Genome Springer-Verlag 3/3(1992-03-01), 156-161 0938-8990 3:3<156 1992 3 335 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer