caa a22 4500 469061936 CHVBK 20180323132900.0 cr unu---uuuuu 170328e19921001xx s 000 0 eng 10.1007/BF00350620 doi (NATIONALLICENCE)springer-10.1007/BF00350620 A mouse genomic library in the bacteriophage P1 cloning system: organization and characterization [Elektronische Daten] [James Pierce, Nat Sternberg, Brian Sauer] Using the bacteriophage P1 cloning system, we have constructed a two to three times coverage, high-molecular-weight (HMW) genomic library from mouse C127 fibroblast cells. The library consists of about 127,500 clones with an average insert size of about 70 kb that are organized into 300 primary pools containing approximately 425 clones per pool. For screening purposes the primary pools are combined into secondary pools (∼4250 clones each) and tertiary pools (∼21,250 clones each). Screening is performed by the polymerase chain reaction (PCR) with DNA isolated from the secondary and tertiary pools. We have screened the library for 13 different mouse sequences and have detected 11. Clones generated from two of the eleven positive screens were isolated from the library (those containing the c-fos and Gαi2 genes) and were further characterized. Direct double-stranded sequencing of DNA from P1 clones with primers bordering the insert provided sequence information from each end of the cloned DNA. Springer-Verlag New York Inc, 1992 Pierce James DuPont Merck Pharmaceutical Company, P.O. Box 80328, 19880-0328, Wilmington, Delaware, USA aut Sternberg Nat DuPont Merck Pharmaceutical Company, P.O. Box 80328, 19880-0328, Wilmington, Delaware, USA aut Sauer Brian DuPont Merck Pharmaceutical Company, P.O. Box 80328, 19880-0328, Wilmington, Delaware, USA aut Mammalian Genome Springer-Verlag 3/10(1992-10-01), 550-558 0938-8990 3:10<550 1992 3 335 https://doi.org/10.1007/BF00350620 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00350620 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pierce James DuPont Merck Pharmaceutical Company, P.O. Box 80328, 19880-0328, Wilmington, Delaware, USA aut NATIONALLICENCE 700 1- Sternberg Nat DuPont Merck Pharmaceutical Company, P.O. Box 80328, 19880-0328, Wilmington, Delaware, USA aut NATIONALLICENCE 700 1- Sauer Brian DuPont Merck Pharmaceutical Company, P.O. Box 80328, 19880-0328, Wilmington, Delaware, USA aut NATIONALLICENCE 773 0- Mammalian Genome Springer-Verlag 3/10(1992-10-01), 550-558 0938-8990 3:10<550 1992 3 335 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer