caa a22 4500 469061960 CHVBK 20180323132900.0 cr unu---uuuuu 170328e19921001xx s 000 0 eng 10.1007/BF00350619 doi (NATIONALLICENCE)springer-10.1007/BF00350619 Modulation of polymorphic loci detection with synthetic tandem repeat variants [Elektronische Daten] [Denis Mariat, Gérard Guérin, Maud Bertaud, Gilles Vergnaud] The modifications of hybridization patterns were studied when Southern blots, carrying stallions DNA samples, were probed with eight synthetic tandem repeats (STRs), related by sequence variations in the basic unit. Because STRs preferentially crosshybridize with genomic VNTRs, they usually give patterns looking more like DNA fingerprints, but we found that even small modifications in the STR monomer could cause major changes in the hybridization profiles and could induce a shift of fingerprint pattern towards the detection of only one or two loci. This enables the use of STRs as direct genetic markers for linkage analysis, without cloning of the corresponding DNA fragment. Moreover, the set of STR variants can suggest consensus sequences allowing some prediction of the banding pattern. Springer-Verlag New York Inc, 1992 Mariat Denis Laboratoire de Génétique Moléculaire, Centre d'Etudes du Bouchet, B.P. N°3, 91710, Vert-Le-Petit, France aut Guérin Gérard Laboratoire de Génétique Biochimique, Institut National de la Recherche Agronomique, 78352, Jouy-en-Josas Cedex, France aut Bertaud Maud Laboratoire de Génétique Biochimique, Institut National de la Recherche Agronomique, 78352, Jouy-en-Josas Cedex, France aut Vergnaud Gilles Laboratoire de Génétique Moléculaire, Centre d'Etudes du Bouchet, B.P. N°3, 91710, Vert-Le-Petit, France aut Mammalian Genome Springer-Verlag 3/10(1992-10-01), 546-549 0938-8990 3:10<546 1992 3 335 https://doi.org/10.1007/BF00350619 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00350619 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mariat Denis Laboratoire de Génétique Moléculaire, Centre d'Etudes du Bouchet, B.P. N°3, 91710, Vert-Le-Petit, France aut NATIONALLICENCE 700 1- Guérin Gérard Laboratoire de Génétique Biochimique, Institut National de la Recherche Agronomique, 78352, Jouy-en-Josas Cedex, France aut NATIONALLICENCE 700 1- Bertaud Maud Laboratoire de Génétique Biochimique, Institut National de la Recherche Agronomique, 78352, Jouy-en-Josas Cedex, France aut NATIONALLICENCE 700 1- Vergnaud Gilles Laboratoire de Génétique Moléculaire, Centre d'Etudes du Bouchet, B.P. N°3, 91710, Vert-Le-Petit, France aut NATIONALLICENCE 773 0- Mammalian Genome Springer-Verlag 3/10(1992-10-01), 546-549 0938-8990 3:10<546 1992 3 335 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer