caa a22 4500 469061987 CHVBK 20180323132900.0 cr unu---uuuuu 170328e19921001xx s 000 0 eng 10.1007/BF00350623 doi (NATIONALLICENCE)springer-10.1007/BF00350623 Deletion mapping of the mouse ornithine decarboxylase-related locus Odc-rs8 within Igh-V [Elektronische Daten] [Beverly Richards-Smith, Peter Brodeur, Rosemary Elliott] The Odc-rs8 locus belongs to a family of mouse DNA sequences related to the gene encoding ornithine decarboxylase (ODC). Odc-rs8 was mapped by recombinant inbred (RI) strain analysis to the region of Chromosome (Chr) 12 occupied by the variable region genes of the immunoglobulin heavy chain (Igh) complex. In the present study, alleles at Odc-rs8 were shown to cosegregate with those for Igh variable region (Igh-V or V H) genes among 37 inbred mouse strains that had been characterized previously for their haplotypes at Igh. For a more precise definition of the location of Odc-rs8 relative to Igh-V, DNAs from 17 Abelson murine leukemia virus (A-MuLV)-transformed pre-B cell lines cultured from mice heterozygous at Igh and Odc-rs8 were analyzed for the presence of DNA restriction fragments (RFs) derived from each parental Odc-rs8 allele. These cell lines, each of which has rearranged one or both Igh genes, previously were employed in mapping members of nine V H gene families by deletion analysis (Brodeur et al. 1988). Comparing the deletion profiles of the cell lines for Odc-rs8 with those for the V H gene families has located Odc-rs8 b within the VHJ558/VH3609 gene cluster and Odc-rs8 c either within or upstream of the 5′-most 9% of VHJ558, identifying Odc-rs8 as a potentially useful marker for the 5′ end of the Igh complex. Springer-Verlag New York Inc, 1992 Richards-Smith Beverly Department of Molecular and Cellular Biology, Roswell Park Cancer Institute, Elm and Carlton Streets, 14263, Buffalo, New York, USA aut Brodeur Peter Department of Pathology, Tufts University School of Medicine, 02111, Boston, Massachusetts, USA aut Elliott Rosemary Department of Molecular and Cellular Biology, Roswell Park Cancer Institute, Elm and Carlton Streets, 14263, Buffalo, New York, USA aut Mammalian Genome Springer-Verlag 3/10(1992-10-01), 568-574 0938-8990 3:10<568 1992 3 335 https://doi.org/10.1007/BF00350623 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00350623 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Richards-Smith Beverly Department of Molecular and Cellular Biology, Roswell Park Cancer Institute, Elm and Carlton Streets, 14263, Buffalo, New York, USA aut NATIONALLICENCE 700 1- Brodeur Peter Department of Pathology, Tufts University School of Medicine, 02111, Boston, Massachusetts, USA aut NATIONALLICENCE 700 1- Elliott Rosemary Department of Molecular and Cellular Biology, Roswell Park Cancer Institute, Elm and Carlton Streets, 14263, Buffalo, New York, USA aut NATIONALLICENCE 773 0- Mammalian Genome Springer-Verlag 3/10(1992-10-01), 568-574 0938-8990 3:10<568 1992 3 335 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer