caa a22 4500 469062266 CHVBK 20180323132901.0 cr unu---uuuuu 170328e19920401xx s 000 0 eng 10.1007/BF00355723 doi (NATIONALLICENCE)springer-10.1007/BF00355723 Modi William Biological Carcinogenesis Development Program, Program Resources, Inc./DynCorp, National Cancer Institute, Frederick Cancer Research and Development Center, 21702-1201, Frederick, Maryland, USA aut Nucleotide sequence and genomic organization of a tandem satellite array from the rock vole Microtus chrotorrhinus (Rodentia) [Elektronische Daten] [William Modi] A tandem satellite array (herein named MSAT-160) has been isolated and characterized from the rodent Microtus chrotorrhinus. Sequence data from 15 partial or complete monomers revealed a repeat unit length of 160 bp. This unit length was apparently derived from two shorter sub-motifs, one a tetramer (GAAA), the other a hexamer (CTTTCT), through polymerase slippage and mutation. Collectively, perfect or imperfect variants of these two motifs comprise nearly 60% of the component. Southern blot analyses of genomic DNA digested with 14 different restriction endonucleases indicated that most enzymes yielded either classical type A or type B restriction patterns, while RsaI yielded a pattern that combined features of both the A and B types, and BamHI appeared to lack sites altogether in MSAT-160. An examination of restriction patterns from 16 individuals with three enzymes failed to identify intraspecific variation, while a related study compared 11 species and documented interspecific distinctiveness (Modi, submitted). Fluorescence in situ hybridization indicated that the satellite DNA was located at the centromeres of several autosomes and at sex chromosome heterochromatin (GenBank accession No. M86843). Springer-Verlag New York Inc., 1992 Mammalian Genome Springer-Verlag 3/4(1992-04-01), 226-232 0938-8990 3:4<226 1992 3 335 https://doi.org/10.1007/BF00355723 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00355723 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Modi William Biological Carcinogenesis Development Program, Program Resources, Inc./DynCorp, National Cancer Institute, Frederick Cancer Research and Development Center, 21702-1201, Frederick, Maryland, USA aut NATIONALLICENCE 773 0- Mammalian Genome Springer-Verlag 3/4(1992-04-01), 226-232 0938-8990 3:4<226 1992 3 335 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer