caa a22 4500 469062320 CHVBK 20180323132901.0 cr unu---uuuuu 170328e19920401xx s 000 0 eng 10.1007/BF00355725 doi (NATIONALLICENCE)springer-10.1007/BF00355725 Somatic cell mapping of the bovine interferon-α receptor [Elektronische Daten] [Jerome Langer, Rengarajulu Puvanakrishnan, James Womack] The bovine interferon-α receptor (BoIFN-αR) mediates the activity of bovine IFN-αs and IFN-β. In addition, human IFN-αs have uniformly high biological activity on bovine cells. A 32P-labeled derivative of human recombinant IFN-αA (HuIFN-αA-P1) binds well and can form a characteristic 130-kDa complex on bovine cells, but not on hamster cells. We have, therefore, analyzed the binding and covalent crosslinking of [32P]HuIFN-αA-P1 to a panel of bovine-hamster somatic cell hybrids. Binding to several bovine-hamster hybrid cell lines was strong (about 30-50% of that seen with bovine MDBK cells) and specific. The binding correlated uniquely with bovine syntenic group U10. In several of the hybrid lines, the ability of human IFN-αB to enhance the expression of endogenous MHC class I molecules correlated with the binding results. We thus conclude that the bovine IFN-αR structural gene (locus designation IFNAR) localizes to syntenic group U10. This group includes a number of other genes whose homologs map to human Chromosome (Chr) 21. Springer-Verlag New York Inc., 1992 Langer Jerome Department of Molecular Genetics and Microbiology, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, 675 Hoes Lane, 08854-5635, Piscataway, New Jersey, USA aut Puvanakrishnan Rengarajulu Department of Molecular Genetics and Microbiology, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, 675 Hoes Lane, 08854-5635, Piscataway, New Jersey, USA aut Womack James Department of Veterinary Pathobiology, Texas A&M University, 77843, College Station, Texas, USA aut Mammalian Genome Springer-Verlag 3/4(1992-04-01), 237-240 0938-8990 3:4<237 1992 3 335 https://doi.org/10.1007/BF00355725 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00355725 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Langer Jerome Department of Molecular Genetics and Microbiology, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, 675 Hoes Lane, 08854-5635, Piscataway, New Jersey, USA aut NATIONALLICENCE 700 1- Puvanakrishnan Rengarajulu Department of Molecular Genetics and Microbiology, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, 675 Hoes Lane, 08854-5635, Piscataway, New Jersey, USA aut NATIONALLICENCE 700 1- Womack James Department of Veterinary Pathobiology, Texas A&M University, 77843, College Station, Texas, USA aut NATIONALLICENCE 773 0- Mammalian Genome Springer-Verlag 3/4(1992-04-01), 237-240 0938-8990 3:4<237 1992 3 335 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer