caa a22 4500 469062347 CHVBK 20180323132901.0 cr unu---uuuuu 170328e19920401xx s 000 0 eng 10.1007/BF00355722 doi (NATIONALLICENCE)springer-10.1007/BF00355722 The structure of the mouse parvalbumin gene [Elektronische Daten] [Martin Schleef, Christine Zühlke, Harald Jockusch, Fritz Schöffl] Parvalbumin (PV) is a calcium-binding protein of the EF-hand family, expressed mainly in fast contracting/relaxing muscles of vertebrates. We have isolated five overlapping genomic PV clones which overall span 28 kilobase pairs (kb) around the Pva locus on mouse Chromosome (Chr) 15. The positions of four introns were determined by DNA sequencing. They interrupt the coding sequences at positions corresponding to those in rat and human PV genes. The transcription start site, 25 bp downstream from the TATA-box, was mapped by oligonucleotide primer extension on poly(A)+-RNA. The analysis of 0.4 kb promoter sequence of the mouse PV gene revealed CCAAT- and TATA-box sequences and a 59 bp GC-rich stretch between positions-59 and-118. Similar motifs have been found in the parvalbumin genes of rat and human. A perfect 11-bp repeat upstream to positions-149 and-163 respectively is homologous only to the rat promoter. These results will be related to tissue and species differences in PV expression. Springer-Verlag New York Inc., 1992 Schleef Martin Developmental Biology Unit W7, University of Bielefeld, P.O. Box 8640, D-W4800, Bielefeld 1, FRG aut Zühlke Christine Human Genetics, University of Göttingen, Goßlerstr. 12d, D-W3400, Göttingen, FRG aut Jockusch Harald Developmental Biology Unit W7, University of Bielefeld, P.O. Box 8640, D-W4800, Bielefeld 1, FRG aut Schöffl Fritz Biological Institute, Genetics, University of Tübingen, Auf der Morgenstelle 28, D-W7400, Tübingen, FRG aut Mammalian Genome Springer-Verlag 3/4(1992-04-01), 217-225 0938-8990 3:4<217 1992 3 335 https://doi.org/10.1007/BF00355722 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00355722 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Schleef Martin Developmental Biology Unit W7, University of Bielefeld, P.O. Box 8640, D-W4800, Bielefeld 1, FRG aut NATIONALLICENCE 700 1- Zühlke Christine Human Genetics, University of Göttingen, Goßlerstr. 12d, D-W3400, Göttingen, FRG aut NATIONALLICENCE 700 1- Jockusch Harald Developmental Biology Unit W7, University of Bielefeld, P.O. Box 8640, D-W4800, Bielefeld 1, FRG aut NATIONALLICENCE 700 1- Schöffl Fritz Biological Institute, Genetics, University of Tübingen, Auf der Morgenstelle 28, D-W7400, Tübingen, FRG aut NATIONALLICENCE 773 0- Mammalian Genome Springer-Verlag 3/4(1992-04-01), 217-225 0938-8990 3:4<217 1992 3 335 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer