caa a22 4500 469062355 CHVBK 20180323132901.0 cr unu---uuuuu 170328e19920401xx s 000 0 eng 10.1007/BF00355719 doi (NATIONALLICENCE)springer-10.1007/BF00355719 Evolution of a B2 tagged sequence from a long-range repeat family in the genus Mus [Elektronische Daten] [Christoph Plass, Thomas Hellwig, Walther Traut, Heinz Winking] A long-range repeat family of more than 50 kb repeat size is clustered in Chromosomes (Chr) 1 of Mus musculus and M. spretus. In M. musculus this long-range repeat family shows considerable variation of copy-number frequency and contains coding regions for at least two genes. In an intron of a gene, which is part of the repeat, a B2 small interspersed repetitive element (SINE) is inserted at identical positions. The B2 element is present in all copies of the long-range repeat family; it was presumably a component of the ancestral single-copy precursor sequence that gave rise by amplification to the repeat family. Copies of the long-range repeat family vary with respect to the number of TAAA tandem repeats in the A-rich 3′ end region of the B2 element. As inferred from polymerase chain reaction (PCR) data, presence and frequency of repeat number variants in the (TAAA)n block are strain and species specific. The B2 element and its flanking regions were sequenced from two copies of the long-range repeat family. Sequence divergence between the two copies (only non-CG base substitutions and deletions/insertions) was determined to be 2.6%. Based on the drift rate in human Alu elements and a correction for the higher drift rates in rodents, and estimate for the divergence time of 1.7 million years was calculated. Since the long-range repeat family is present in M. musculus and M. spretus, it must have evolved by amplification before the separation of the two species about 1-4 million years ago. Springer-Verlag New York Inc., 1992 Plass Christoph Institut für Biologie, Medizinische Universität zu Lübeck, Ratzeburger Allee 160, D-2400, Lübeck, FRG aut Hellwig Thomas Institut für Biologie, Medizinische Universität zu Lübeck, Ratzeburger Allee 160, D-2400, Lübeck, FRG aut Traut Walther Institut für Biologie, Medizinische Universität zu Lübeck, Ratzeburger Allee 160, D-2400, Lübeck, FRG aut Winking Heinz Institut für Biologie, Medizinische Universität zu Lübeck, Ratzeburger Allee 160, D-2400, Lübeck, FRG aut Mammalian Genome Springer-Verlag 3/4(1992-04-01), 197-201 0938-8990 3:4<197 1992 3 335 https://doi.org/10.1007/BF00355719 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00355719 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Plass Christoph Institut für Biologie, Medizinische Universität zu Lübeck, Ratzeburger Allee 160, D-2400, Lübeck, FRG aut NATIONALLICENCE 700 1- Hellwig Thomas Institut für Biologie, Medizinische Universität zu Lübeck, Ratzeburger Allee 160, D-2400, Lübeck, FRG aut NATIONALLICENCE 700 1- Traut Walther Institut für Biologie, Medizinische Universität zu Lübeck, Ratzeburger Allee 160, D-2400, Lübeck, FRG aut NATIONALLICENCE 700 1- Winking Heinz Institut für Biologie, Medizinische Universität zu Lübeck, Ratzeburger Allee 160, D-2400, Lübeck, FRG aut NATIONALLICENCE 773 0- Mammalian Genome Springer-Verlag 3/4(1992-04-01), 197-201 0938-8990 3:4<197 1992 3 335 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer