caa a22 4500 469062533 CHVBK 20180323132901.0 cr unu---uuuuu 170328e19920501xx s 000 0 eng 10.1007/BF00292155 doi (NATIONALLICENCE)springer-10.1007/BF00292155 The mouse male germ cell-specific gene Tpx-1 [Elektronische Daten] molecular structure, mode of expression in spermatogenesis, and sequence similarity to two non-mammalian genes [Nobuyuki Mizuki, Donna Sarapata, Jose Garcia-Sanz, Masanori Kasahara] Tpx-1 is a testis-specific gene that maps on mouse Chromosome (Chr) 17. The deduced TPX-1 protein shows 55% amino acid sequence similarity to acidic epididymal glycoprotein (AEG), assumed to be involved in sperm maturation. In the present study, we determined the genomic structure of the mouse Tpx-1 gene and the cellular localization of its transcripts. The gene was found to contain ten exons, with an unusually large intron (∼17.0 kilobase pairs) between exons 8 and 9. In situ hybridization of testicular sections showed that Tpx-1 is transcribed abundantly by haploid male germ cells. A computer search of protein databases revealed that deduced TPX-1/AEG proteins have significant sequence similarity (∼30%) to two non-mammalian proteins: "pathogenesis-related” proteins 1 of tobaccos, and venom sac proteins of whiteface hornets, known as Dol m V. Amino acid residues encoded by exon 10 of the Tpx-1 gene and most of those encoded by exon 9 were absent in the non-mammalian proteins. This result suggests that the ancestor of Tpx-1 acquired exons 9 and 10 after its divergence from the ancestors of the plant and insect proteins. Springer-Verlag New York Inc, 1992 Mizuki Nobuyuki Department of Microbiology and Immunology, University of Miami School of Medicine, P.O. Box 016960 (R138), 33101, Miami, Florida, USA aut Sarapata Donna Department of Microbiology and Immunology, University of Miami School of Medicine, P.O. Box 016960 (R138), 33101, Miami, Florida, USA aut Garcia-Sanz Jose Department of Microbiology and Immunology, University of Miami School of Medicine, P.O. Box 016960 (R138), 33101, Miami, Florida, USA aut Kasahara Masanori Department of Microbiology and Immunology, University of Miami School of Medicine, P.O. Box 016960 (R138), 33101, Miami, Florida, USA aut Mammalian Genome Springer-Verlag 3/5(1992-05-01), 274-280 0938-8990 3:5<274 1992 3 335 https://doi.org/10.1007/BF00292155 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00292155 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mizuki Nobuyuki Department of Microbiology and Immunology, University of Miami School of Medicine, P.O. Box 016960 (R138), 33101, Miami, Florida, USA aut NATIONALLICENCE 700 1- Sarapata Donna Department of Microbiology and Immunology, University of Miami School of Medicine, P.O. Box 016960 (R138), 33101, Miami, Florida, USA aut NATIONALLICENCE 700 1- Garcia-Sanz Jose Department of Microbiology and Immunology, University of Miami School of Medicine, P.O. Box 016960 (R138), 33101, Miami, Florida, USA aut NATIONALLICENCE 700 1- Kasahara Masanori Department of Microbiology and Immunology, University of Miami School of Medicine, P.O. Box 016960 (R138), 33101, Miami, Florida, USA aut NATIONALLICENCE 773 0- Mammalian Genome Springer-Verlag 3/5(1992-05-01), 274-280 0938-8990 3:5<274 1992 3 335 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer