caa a22 4500 469062568 CHVBK 20180323132901.0 cr unu---uuuuu 170328e19920501xx s 000 0 eng 10.1007/BF00292154 doi (NATIONALLICENCE)springer-10.1007/BF00292154 Characterization of murine carcinoembryonic antigen gene family members [Elektronische Daten] [Fritz Rudert, Ann Saunders, Sabine Rebstock, John Thompson, Wolfgang Zimmermann] The carcinoembryonic antigen (CEA) is a human tumor marker whose gene belongs to a family with more than 20 members. This gene family codes for a group of proteins with in vitro cell adhesion properties and for a group of abundantly expressed pregnancy-specific glycoproteins (PSG) with unknown functions. As a basis for in vivo functional studies, we have started to analyze the murine CEA gene family and have identified five new members (Cea-2 to Cea-6). cDNA clones were isolated for Cea-2, Cea-3, and Cea-6. The deduced amino acid sequences of Cea-2 and Cea-6 indicate three IgV-like (N), followed by one IgC-like (A) domain (N1-N2-N3-A). We have also partially characterized the Cea-2 gene and two additional ones, Cea-4 and Cea-5. Cea-2 and Cea-4 are separated by only 16 kb, suggesting a close linkage of murine CEA-related genes, as found for the human CEA gene family. Cea-5 was located to the proximal region of mouse Chromosome (Chr) 7, which is syntenic to part of human Chr 19, containing the human CEA gene family cluster. Cea-2, Cea-3, and a Cea-4-like gene are differentially transcribed in the placenta during pregnancy, but not in other organs tested. This expression pattern strongly suggests that they represent counterparts of the human PSG subgroup members, despite the presence of multiple IgV-like domains, a feature not found for human PSGs. The more distantly related Cea-5 seems to be ubiquitously expressed. The putative promoter region of Cea-2 lacks typical TATA-or CAAT-boxes, but contains other conserved motifs that could play a role in the initiation of transcription. Springer-Verlag New York Inc, 1992 Rudert Fritz Institut für Immunbiologie, Universität Freiburg, Stefan-Meier-Strasse 8, D-7800, Freiburg, FRG aut Saunders Ann Division of Neurology, Duke University Medical Center, 27710, Durham, North Carolina, USA aut Rebstock Sabine Institut für Immunbiologie, Universität Freiburg, Stefan-Meier-Strasse 8, D-7800, Freiburg, FRG aut Thompson John Institut für Immunbiologie, Universität Freiburg, Stefan-Meier-Strasse 8, D-7800, Freiburg, FRG aut Zimmermann Wolfgang Institut für Immunbiologie, Universität Freiburg, Stefan-Meier-Strasse 8, D-7800, Freiburg, FRG aut Mammalian Genome Springer-Verlag 3/5(1992-05-01), 262-273 0938-8990 3:5<262 1992 3 335 https://doi.org/10.1007/BF00292154 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00292154 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rudert Fritz Institut für Immunbiologie, Universität Freiburg, Stefan-Meier-Strasse 8, D-7800, Freiburg, FRG aut NATIONALLICENCE 700 1- Saunders Ann Division of Neurology, Duke University Medical Center, 27710, Durham, North Carolina, USA aut NATIONALLICENCE 700 1- Rebstock Sabine Institut für Immunbiologie, Universität Freiburg, Stefan-Meier-Strasse 8, D-7800, Freiburg, FRG aut NATIONALLICENCE 700 1- Thompson John Institut für Immunbiologie, Universität Freiburg, Stefan-Meier-Strasse 8, D-7800, Freiburg, FRG aut NATIONALLICENCE 700 1- Zimmermann Wolfgang Institut für Immunbiologie, Universität Freiburg, Stefan-Meier-Strasse 8, D-7800, Freiburg, FRG aut NATIONALLICENCE 773 0- Mammalian Genome Springer-Verlag 3/5(1992-05-01), 262-273 0938-8990 3:5<262 1992 3 335 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer