caa a22 4500 469062592 CHVBK 20180323132902.0 cr unu---uuuuu 170328e19920201xx s 000 0 eng 10.1007/BF00431248 doi (NATIONALLICENCE)springer-10.1007/BF00431248 Polymorphisms revealed by PCR with single, short-sized, arbitrary primers are reliable markers for mouse and rat gene mapping [Elektronische Daten] [Tadao Serikawa, Xavier Montagutelli, Dominique Simon-Chazottes, Jean-Louis Guénet] Ten single, arbitrarily designed oligodeoxynucleotide primers, with 50-70% (G+C) content, were used to amplify by polymerase chain reaction (PCR) sequences with DNA templates from several mouse species (Mus spretus, Mus musculus musculus, and Mus musculus domesticus), as well as DNA from the laboratory rat (Rattus norvegicus). Eight of these ten primers, used either individually or associated in pairs, generated a total of 13 polymorphic products which were used as genetic markers. All of these polymorphic sequences but one were mapped to a particular mouse chromosome, by use of DNA panels prepared either from interspecific backcross progeny of the type (C57BL/6 x Mus spretus)F1 x C57BL/6 or DNA samples prepared from two sets of recombinant inbred (RI) strains (AKXL and BXD). Six rat-specific DNA segments were also assigned to a particular chromosome with DNA panels prepared from 18 rat/mouse somatic cell hybrids segregating rat chromosomes. From these experiments we conclude that, under precisely standardized PCR conditions, the DNA molecules amplified with these arbitrarily designed primers are useful and reliable markers for genetic mapping in both mouse and rat. Springer-Verlag New York Inc, 1992 Serikawa Tadao Unité de Génétique des Mammifères de l'Institut Pasteur, 25 rue du Docteur Roux, 75724, Paris, Cedex 15, France aut Montagutelli Xavier Unité de Génétique des Mammifères de l'Institut Pasteur, 25 rue du Docteur Roux, 75724, Paris, Cedex 15, France aut Simon-Chazottes Dominique Unité de Génétique des Mammifères de l'Institut Pasteur, 25 rue du Docteur Roux, 75724, Paris, Cedex 15, France aut Guénet Jean-Louis Unité de Génétique des Mammifères de l'Institut Pasteur, 25 rue du Docteur Roux, 75724, Paris, Cedex 15, France aut Mammalian Genome Springer-Verlag 3/2(1992-02-01), 65-72 0938-8990 3:2<65 1992 3 335 https://doi.org/10.1007/BF00431248 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00431248 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Serikawa Tadao Unité de Génétique des Mammifères de l'Institut Pasteur, 25 rue du Docteur Roux, 75724, Paris, Cedex 15, France aut NATIONALLICENCE 700 1- Montagutelli Xavier Unité de Génétique des Mammifères de l'Institut Pasteur, 25 rue du Docteur Roux, 75724, Paris, Cedex 15, France aut NATIONALLICENCE 700 1- Simon-Chazottes Dominique Unité de Génétique des Mammifères de l'Institut Pasteur, 25 rue du Docteur Roux, 75724, Paris, Cedex 15, France aut NATIONALLICENCE 700 1- Guénet Jean-Louis Unité de Génétique des Mammifères de l'Institut Pasteur, 25 rue du Docteur Roux, 75724, Paris, Cedex 15, France aut NATIONALLICENCE 773 0- Mammalian Genome Springer-Verlag 3/2(1992-02-01), 65-72 0938-8990 3:2<65 1992 3 335 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer