caa a22 4500 469062657 CHVBK 20180323132902.0 cr unu---uuuuu 170328e19920201xx s 000 0 eng 10.1007/BF00431247 doi (NATIONALLICENCE)springer-10.1007/BF00431247 Multilocus markers for mouse genome analysis: PCR amplification based on single primers of arbitrary nucleotide sequence [Elektronische Daten] [Joseph Nadeau, Hendrick Bedigian, Gayle Bouchard, Térèse Denial, Michael Kosowsky, Robert Norberg, Sherri Pugh, Evelyn Sargeant, Rowena turner, Beverly Paigen] Polymerase chain reaction (PCR) based on single primers of arbitrary nucleotide sequence provides a powerful marker system for genome analysis because each primer amplifies multiple products, and cloning, sequencing, and hybridization are not required. We have evaluated this typing system for the mouse by identifying optimal PCR conditions; characterizing effects of GC content, primer length, and multiplexed primers; demonstrating considerable variation among a panel of inbred strains; and establishing linkage for several products. Mg2+, primer, template, and annealing conditions were identified that optimized the number and resolution of amplified products. Primers with 40% GC content failed to amplify products readily, primers with 50% GC content resulted in reasonable amplification, and primers with 60% GC content gave the largest number of well-resolved products. Longer primers did not necessarily amplify more products than shorter primers of the same proportional GC content. Multiplexed primers yielded more products than either primer alone and usually revealed novel variants. A strain survey showed that most strains could be readily distinguished with a modest number of primers. Finally, linkage for seven products was established on five chromosomes. These characteristics establish single primer PCR as a powerful method for mouse genome analysis. Springer-Verlag New York Inc, 1992 Nadeau Joseph The Jackson Laboratory, 04609, Bar Harbor, Maine, USA aut Bedigian Hendrick The Jackson Laboratory, 04609, Bar Harbor, Maine, USA aut Bouchard Gayle The Jackson Laboratory, 04609, Bar Harbor, Maine, USA aut Denial Térèse The Jackson Laboratory, 04609, Bar Harbor, Maine, USA aut Kosowsky Michael The Jackson Laboratory, 04609, Bar Harbor, Maine, USA aut Norberg Robert The Jackson Laboratory, 04609, Bar Harbor, Maine, USA aut Pugh Sherri The Jackson Laboratory, 04609, Bar Harbor, Maine, USA aut Sargeant Evelyn The Jackson Laboratory, 04609, Bar Harbor, Maine, USA aut turner Rowena The Jackson Laboratory, 04609, Bar Harbor, Maine, USA aut Paigen Beverly The Jackson Laboratory, 04609, Bar Harbor, Maine, USA aut Mammalian Genome Springer-Verlag 3/2(1992-02-01), 55-64 0938-8990 3:2<55 1992 3 335 https://doi.org/10.1007/BF00431247 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00431247 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nadeau Joseph The Jackson Laboratory, 04609, Bar Harbor, Maine, USA aut NATIONALLICENCE 700 1- Bedigian Hendrick The Jackson Laboratory, 04609, Bar Harbor, Maine, USA aut NATIONALLICENCE 700 1- Bouchard Gayle The Jackson Laboratory, 04609, Bar Harbor, Maine, USA aut NATIONALLICENCE 700 1- Denial Térèse The Jackson Laboratory, 04609, Bar Harbor, Maine, USA aut NATIONALLICENCE 700 1- Kosowsky Michael The Jackson Laboratory, 04609, Bar Harbor, Maine, USA aut NATIONALLICENCE 700 1- Norberg Robert The Jackson Laboratory, 04609, Bar Harbor, Maine, USA aut NATIONALLICENCE 700 1- Pugh Sherri The Jackson Laboratory, 04609, Bar Harbor, Maine, USA aut NATIONALLICENCE 700 1- Sargeant Evelyn The Jackson Laboratory, 04609, Bar Harbor, Maine, USA aut NATIONALLICENCE 700 1- turner Rowena The Jackson Laboratory, 04609, Bar Harbor, Maine, USA aut NATIONALLICENCE 700 1- Paigen Beverly The Jackson Laboratory, 04609, Bar Harbor, Maine, USA aut NATIONALLICENCE 773 0- Mammalian Genome Springer-Verlag 3/2(1992-02-01), 55-64 0938-8990 3:2<55 1992 3 335 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer