caa a22 4500 469062665 CHVBK 20180323132902.0 cr unu---uuuuu 170328e19920201xx s 000 0 eng 10.1007/BF00431252 doi (NATIONALLICENCE)springer-10.1007/BF00431252 Molecular analysis of human Chromosome 16 cosmid clones containing Not I sites [Elektronische Daten] [Terry Lerner, Gabriella Wright, Benjamin Leverone, William Dackowski, Donna Shook, Mary Anderson, Katherine Klinger, David Callen, Gregory Landes] To test the feasibility of using cloned NotI sites as markers for physical mapping, we have screened for cosmid clones spanning the NotI sites on human Chromosome (Chr) 16. Fluorescence in situ hybridization analysis of these clones confirms the previously reported cluster of NotI sites on 16p13.3. Methylation status of the cloned NotI sites on genomic DNA was established by hybridization of the cosmids to Southern blots containing EcoRI and EcoRI/NotI digest of genomic DNA. These results indicated that four of six clones included in our study can be used as linking clones for physical mapping. Two clones have NotI sites which are not cleavable in the cell lines tested. In one clone, the NotI site exists as an isolated rare-cutting restriction enzyme site, whereas in the other clone the NotI site appears to be island-related. Springer-Verlag New York Inc, 1992 Lerner Terry Integrated Genetics, Inc., 01701, Framingham, Massachusetts, USA aut Wright Gabriella Integrated Genetics, Inc., 01701, Framingham, Massachusetts, USA aut Leverone Benjamin Integrated Genetics, Inc., 01701, Framingham, Massachusetts, USA aut Dackowski William Integrated Genetics, Inc., 01701, Framingham, Massachusetts, USA aut Shook Donna Integrated Genetics, Inc., 01701, Framingham, Massachusetts, USA aut Anderson Mary Integrated Genetics, Inc., 01701, Framingham, Massachusetts, USA aut Klinger Katherine Integrated Genetics, Inc., 01701, Framingham, Massachusetts, USA aut Callen David Adelaide Children's Hospital, Adelaide, Australia aut Landes Gregory Integrated Genetics, Inc., 01701, Framingham, Massachusetts, USA aut Mammalian Genome Springer-Verlag 3/2(1992-02-01), 92-100 0938-8990 3:2<92 1992 3 335 https://doi.org/10.1007/BF00431252 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00431252 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lerner Terry Integrated Genetics, Inc., 01701, Framingham, Massachusetts, USA aut NATIONALLICENCE 700 1- Wright Gabriella Integrated Genetics, Inc., 01701, Framingham, Massachusetts, USA aut NATIONALLICENCE 700 1- Leverone Benjamin Integrated Genetics, Inc., 01701, Framingham, Massachusetts, USA aut NATIONALLICENCE 700 1- Dackowski William Integrated Genetics, Inc., 01701, Framingham, Massachusetts, USA aut NATIONALLICENCE 700 1- Shook Donna Integrated Genetics, Inc., 01701, Framingham, Massachusetts, USA aut NATIONALLICENCE 700 1- Anderson Mary Integrated Genetics, Inc., 01701, Framingham, Massachusetts, USA aut NATIONALLICENCE 700 1- Klinger Katherine Integrated Genetics, Inc., 01701, Framingham, Massachusetts, USA aut NATIONALLICENCE 700 1- Callen David Adelaide Children's Hospital, Adelaide, Australia aut NATIONALLICENCE 700 1- Landes Gregory Integrated Genetics, Inc., 01701, Framingham, Massachusetts, USA aut NATIONALLICENCE 773 0- Mammalian Genome Springer-Verlag 3/2(1992-02-01), 92-100 0938-8990 3:2<92 1992 3 335 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer