caa a22 4500 469062681 CHVBK 20180323132902.0 cr unu---uuuuu 170328e19920201xx s 000 0 eng 10.1007/BF00431254 doi (NATIONALLICENCE)springer-10.1007/BF00431254 Assignment of eight loci to bovine syntenic groups by use of PCR: extension of a comparative gene map [Elektronische Daten] [Allan Dietz, Holly Neibergs, James Womack] The polymerase chain reaction (PCR) has been combined with hybrid somatic cell technology to extend the bovine physical map. Eight bovine loci—glycoprotein hormone alpha (CGA), coagulation factor X (F10), chromogranin A (CHGA), low-density lipoprotein receptor (LDLR), human prochymosin pseudogene (CYM), oxytocin (OXT), arginine-vasopressin (ARVP), and cytochrome oxidase c subunit IV pseudogene (COXP)—were assigned to bovine syntenic groups with this approach. CGA was assigned to bovine syntenic group U2, F10 to U27, CHGA to U4 [bovine Chromosome (Chr) 21], LDLR to U22, CYM to U6, OXT and ARVP to U11, and COXP to U3 (bovine Chr 5). Seven of these genes, CGA, F10, CHGA, LDLR, OXT, ARVP, and CYM, further delineate regions of chromosomal conservation on human Chrs 6, 13, 14, 19, 20, 20, and 1, respectively. CHGA, OXT, and ARVP are unmapped in the mouse. Comparative mapping predicts the mouse CHGA will map to Chr 12, and mouse OXT and ARVP will map to mouse Chr 2. Furthermore, human CYM is predicted to be sublocalized to 1p32-q21. The primers developed for these eight loci will be useful for the development of hybrid somatic cell panels in the future as well as establishing a collection of bovine expressed sequence tags. Springer-Verlag New York Inc, 1992 Dietz Allan Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, 77843, College Station, Texas, USA aut Neibergs Holly Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, 77843, College Station, Texas, USA aut Womack James Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, 77843, College Station, Texas, USA aut Mammalian Genome Springer-Verlag 3/2(1992-02-01), 106-111 0938-8990 3:2<106 1992 3 335 https://doi.org/10.1007/BF00431254 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00431254 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dietz Allan Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, 77843, College Station, Texas, USA aut NATIONALLICENCE 700 1- Neibergs Holly Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, 77843, College Station, Texas, USA aut NATIONALLICENCE 700 1- Womack James Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, 77843, College Station, Texas, USA aut NATIONALLICENCE 773 0- Mammalian Genome Springer-Verlag 3/2(1992-02-01), 106-111 0938-8990 3:2<106 1992 3 335 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer