caa a22 4500 469062789 CHVBK 20180323132902.0 cr unu---uuuuu 170328e19921101xx s 000 0 eng 10.1007/BF00352477 doi (NATIONALLICENCE)springer-10.1007/BF00352477 Hybridization fingerprinting of high-density cDNA-library arrays with cDNA pools derived from whole tissues [Elektronische Daten] [Thomas Gress, Jörg Hoheisel, Gregory Lennon, Günther Zehetner, Hans Lehrach] As part of an integrated mapping and sequencing analysis of genomes, we have developed an approach allowing the characterization of large numbers of cDNA library clones with a minimal number of experiments. Three basic elements used in the analysis of cDNA libraries are responsible for the high efficiency of this new approach: (1) high-density library arrays allowing thousands of clones to be screened simultaneously; (2) hybridization fingerprinting techniques to identify clones abundantly expressed in specific tissues (by hybridizations with labeled tissue cDNA pools) and to avoid the repeated selection of identical clones and of clones containing noncoding inserts; and (3) a computerized system for the evaluation of hybridization data. To demonstrate the feasibility of this approach, we hybridized high-density cDNA library arrays of human fetal brain and embryonal Drosophila with radiolabeled cDNA pools derived from whole mouse tissues. Fingerprints of the library arrays were generated, localizing clones containing cDNA sequences from mRNAs expressed at middle to high abundance (>0.1-0.15%) in the respective tissue. Partial sequencing data from a number of clones abundantly expressed in several tissues were generated to demonstrate the value of the approach, especially for the selection of cDNA clones for the analyses of genomes based on expressed sequence tagged sites. Data obtained by the technique described will ultimately be correlated with additional transcriptional and sequence information for the same library clones and with genomic mapping information in a relational database. Springer-Verlag New York Inc, 1992 Gress Thomas Imperial Cancer Research Fund, 44 Lincoln's Inn Fields, P.O. Box 123, WC2a 3PX, London, UK aut Hoheisel Jörg Imperial Cancer Research Fund, 44 Lincoln's Inn Fields, P.O. Box 123, WC2a 3PX, London, UK aut Lennon Gregory Imperial Cancer Research Fund, 44 Lincoln's Inn Fields, P.O. Box 123, WC2a 3PX, London, UK aut Zehetner Günther Imperial Cancer Research Fund, 44 Lincoln's Inn Fields, P.O. Box 123, WC2a 3PX, London, UK aut Lehrach Hans Imperial Cancer Research Fund, 44 Lincoln's Inn Fields, P.O. Box 123, WC2a 3PX, London, UK aut Mammalian Genome Springer-Verlag 3/11(1992-11-01), 609-619 0938-8990 3:11<609 1992 3 335 https://doi.org/10.1007/BF00352477 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00352477 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gress Thomas Imperial Cancer Research Fund, 44 Lincoln's Inn Fields, P.O. Box 123, WC2a 3PX, London, UK aut NATIONALLICENCE 700 1- Hoheisel Jörg Imperial Cancer Research Fund, 44 Lincoln's Inn Fields, P.O. Box 123, WC2a 3PX, London, UK aut NATIONALLICENCE 700 1- Lennon Gregory Imperial Cancer Research Fund, 44 Lincoln's Inn Fields, P.O. Box 123, WC2a 3PX, London, UK aut NATIONALLICENCE 700 1- Zehetner Günther Imperial Cancer Research Fund, 44 Lincoln's Inn Fields, P.O. Box 123, WC2a 3PX, London, UK aut NATIONALLICENCE 700 1- Lehrach Hans Imperial Cancer Research Fund, 44 Lincoln's Inn Fields, P.O. Box 123, WC2a 3PX, London, UK aut NATIONALLICENCE 773 0- Mammalian Genome Springer-Verlag 3/11(1992-11-01), 609-619 0938-8990 3:11<609 1992 3 335 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer